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THEMIS Antibody

  • 中文名称：
    THEMIS兔多克隆抗体
  • 货号：
    CSB-PA850780LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Jurkat whole cell lysate, K562 whole cell lysate
      All lanes: THEMIS antibody at 7.6µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 74, 70, 63, 78 kDa
      Observed band size: 74, 70 kDa
    • IHC image of CSB-PA850780LA01HU diluted at 1:300 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA850780LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) THEMIS Polyclonal antibody
  • Uniprot No.：
    Q8N1K5
  • 基因名：
    THEMIS
  • 别名：
    C6orf190 antibody; C6orf207 antibody; MGC163388 antibody; Protein THEMIS antibody; SIGNALING PHOSPHOPROTEIN SPECIFIC FOR T CELLS antibody; SPOT antibody; THEMIS antibody; THMS1_HUMAN antibody; Thymocyte expressed molecule involved in selection antibody; Thymocyte selection associated antibody; Thymocyte selection pathway associated antibody; Thymocyte-expressed molecule involved in selection antibody; TSEPA antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Protein THEMIS protein (547-641AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，THEMIS Antibody (CSB-PA850780LA01HU)，的标记方式是Non-conjugated。对于THEMIS Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA850780LB01HU THEMIS Antibody, HRP conjugated ELISA
    FITC CSB-PA850780LC01HU THEMIS Antibody, FITC conjugated
    Biotin CSB-PA850780LD01HU THEMIS Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk.
  • 基因功能参考文献：
    1. we have demonstrated mutually exclusive use of exon 1 and 2 amongst 16 novel THEMIS isoforms. We also show inverse correlation between THEMIS expression in human CD4+ T-cells and dosage of the multiple sclerosis risk allele at rs13204742, driven by reduced expression of exon 1- containing isoforms PMID: 27438997
    2. we found that THEMIS directly regulated the catalytic activity of the tyrosine phosphatase SHP-1. PMID: 28250424
    3. the THEMIS:SHP complex dampens early TCR signalling by a previously unknown molecular mechanism that favours T-cell survival. PMID: 25535246
    4. THEMIS showed higher mRNA expression in active celiac disease compared with treated patients and controls. PMID: 23820479
    5. THEMIS binding to GRB2 was required for thymocyte development. PMID: 23460737
    6. Themis1 exhibits the same potential to restore T cell development and T cell receptor-mediated signaling in Themis1-deficient mice. PMID: 22732588
    7. SPOP mutations may define a new molecular subtype of prostate cancer. PMID: 22610119
    8. specific role for THEMIS in early TCR signalosome function PMID: 21189249
    9. Observational study of gene-disease association. (HuGE Navigator) PMID: 19913121
    10. Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 20628086
    11. Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20095854
    12. Functional characterization of the homologous mouse gene. PMID: 19597498
    13. Functional analysis of the mouse gene by knockout, and comparison of the mouse and human proteins. PMID: 19597499

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Themis family
  • 数据库链接：

    HGNC: 21569

    OMIM: 613607

    KEGG: hsa:387357

    UniGene: Hs.661756