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SSBP1 Antibody

  • 货号：
    CSB-PA589424
  • 规格：
    ￥2024
  • 图片：
    • Western blot analysis of extracts from HeLa cells and HUVEC cells, using MtSSB antibody.
    • Immunohistochemistry analysis of paraffin-embedded human pancreas tissue using MtSSB antibody.
    • Immunofluorescence analysis of A549 cells, using MtSSB antibody.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) SSBP1 Polyclonal antibody
  • Uniprot No.：
    Q04837
  • 基因名：
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Synthesized peptide derived from internal of Human MtSSB.
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB,IHC,IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:3000
    IHC 1:50-1:100
    IF 1:100-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Binds preferentially and cooperatively to pyrimidine rich single-stranded DNA (ss-DNA). In vitro, required to maintain the copy number of mitochondrial DNA (mtDNA) and plays a crucial role during mtDNA replication by stimulating the activity of the replisome components POLG and TWNK at the replication fork. Promotes the activity of the gamma complex polymerase POLG, largely by organizing the template DNA and eliminating secondary structures to favor ss-DNA conformations that facilitate POLG activity. In addition it is able to promote the 5'-3' unwinding activity of the mtDNA helicase TWNK. May also function in mtDNA repair.
  • 基因功能参考文献：
    1. This study identified SSBP1 as a putative N6-methyldeoxyadenosine binding protein. PMID: 30412255
    2. NEIL1 and homotetrameric mtSSB form a larger ternary complex in presence of DNA, however, the tetrameric form of mtSSB gets disrupted by NEIL1 in the absence of DNA as revealed by the formation of a smaller NEIL1-mtSSBmonomer complex. PMID: 29522991
    3. These results indicated that SSDBP1 may induce cell chemoresistance of cisplatin through promoting DNA repair, resistance-related gene expression, cell proliferation, and inhibiting apoptosis. PMID: 28210897
    4. Data show that single-stranded DNA binding protein 1 (hSSB1/NABP2/OBFC2B) forms a tetramer and is stabilized by two cysteines (C81 and C99) as well as a subset of charged and hydrophobic residues. PMID: 28609781
    5. DNA synthesis determines the binding mode of the human mtSSB. PMID: 28486639
    6. The data presented in this study suggests that while SSBP1 has predominantly been considered in a DNA repair context, it is likely to have roles in many other cellular processes. In particular, the identification of numerous proteins with roles in mRNA metabolism, transcriptional transactions and ribosomal processes, may suggest these as important areas of SSBP1 function. PMID: 27938330
    7. Data suggest that human mitochondrial SSB shares many physicochemical properties with E coli SSB and that the differences may be explained by the lack of an acidic, disordered C-terminal tail in human mitochondrial SSB protein. (SSB = single-stranded DNA-binding protein) PMID: 28615444
    8. Data suggest that human mitochondrial SSB and E coli SSB both protect and expose single-stranded DNA; implications from these studies toward understanding DNA replication and DNA repair abound. (SSB = single-stranded DNA-binding protein) [EDITORIAL] PMID: 28778884
    9. hSSB1 is directly phosphorylated by DNA-PK at serine residue 134. While this modification is largely suppressed in undamaged cells by PPP-family protein phosphatases, S134 phosphorylation is enhanced following the disruption of replication forks and promotes cellular survival. PMID: 28448822
    10. The stimulatory effect of mtSSB on Pol gamma on these ssDNA templates is not species-specific. PMID: 26446790
    11. This study determined that hSSB1 acetylation at K94 mediated by the acetyltransferase p300 and the deacetylases SIRT4 and HDAC10 impaired its ubiquitin-mediated degradation by proteasomes. PMID: 26170237
    12. SSBP1 protects cells from proteotoxic stresses by potentiating stress-induced HSF1 transcriptional activity PMID: 25762445
    13. Fbxl5 interacts with and targets hSSB1 for ubiquitination and degradation, which could be prevented by ATM-mediated hSSB1 T117 phosphorylation. PMID: 25249620
    14. In response to the DNA damage response, INTS3-hSSB1-INTS6 complex relocates to the DNA damage sites. PMID: 23986477
    15. Results indicate that hSSB1 may regulate DNA damage checkpoints by positively modulating p53 and its downstream target p21. PMID: 23184057
    16. mtSSB potentially prevents formation of DNA breaks in ssDNA, ensuring that base removal primarily occurs in dsDNA. PMID: 23290262
    17. Local enrichment of UNG1 at DNA-bound mtSSB may furthermore facilitate rapid access to- and processing of the damage once the dsDNA conformation is restored. PMID: 22153281
    18. mtSSB uses distinct structural elements to interact functionally with its mtDNA replisome partners and to promote proper mtDNA replication in animal cells PMID: 21953457
    19. hSSB1 may positively modulate p21 to regulate cell cycle progression and DNA damage response, implicating hSSB1 as a novel, promising therapeutic target for cancers such as hepatocellular carcinoma PMID: 21242961
    20. Results describe the role of mitochondrial single-stranded DNA binding protein in the maintenance of mitochondrial DNA in cultured human cells. PMID: 20434493
    21. Data show that mtSSB bound both CKS1 and CKS2 and underwent CDK-dependent phosphorylation. PMID: 19786724
    22. SSDP1 gene encodes a protein with a conserved N-terminal FORWARD domain;The SSDP1 mRNA transcripts are distributed ubiquitously in adult human and mouse tissues. PMID: 12479417
    23. PDIP38 is located in the mitochondrial matrix. TFAM and mitochondrial single-stranded DNA binding protein (mtSSB) are co-immunoprecipitated with PDIP38 PMID: 16428295
    24. Zta interaction with mtSSB serves the dual function of facilitating viral and blocking mitochondrial DNA replication. PMID: 18305033
    25. Identification of human mitochondrial SSB (HmtSSB) as a novel protein-binding partner of tumour suppressor p53, in mitochondria. PMID: 19066201
    26. hSSB1 and hSSB2 form two separate complexes with similar structures, and both complexes participate in DNA damage response. PMID: 19605351
    27. following DNA damage, SSB1 and Integrator3 relocate to the DNA damage sites and regulate the accumulation of TopBP1 and BRCA1 there PMID: 19759019

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  • 亚细胞定位：
    Mitochondrion. Mitochondrion matrix, mitochondrion nucleoid.
  • 组织特异性：
    Expressed in retinal ganglion cells, photoreceptors, pigmented epithelium and fibroblasts (at protein level).
  • 数据库链接：

    HGNC: 11317

    OMIM: 600439

    KEGG: hsa:6742

    STRING: 9606.ENSP00000265304

    UniGene: Hs.490394