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  4. SPY Antibody

SPY Antibody

  • 中文名称：
    SPY兔多克隆抗体
  • 货号：
    CSB-PA300608XA01ENV
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in Recombinant protein
      All lanes: SPY antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 26.8 kDa
      Observed band size: 33 kDa
  • 其他：

产品详情

  • Uniprot No.：
    P77754
  • 基因名：
    SPY
  • 别名：
    Periplasmic chaperone Spy (Spheroplast protein Y) spy b1743 JW1732
  • 反应种属：
    Escherichia coli (strain K12)
  • 免疫原：
    Recombinant Escherichia coli (strain K12) SPY protein (52-147aa)
  • 免疫原种属：
    Escherichia coli (strain K12)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Protein G
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    An ATP-independent periplasmic chaperone, decreases protein aggregation and helps protein refolding. Binds substrate over a large region of its convex inner surface. Substrate protein folds while it is bound to chaperone. Increasing Spy flexibility increases its substrate affinity and overall chaperone activity (shown for 3 different substrates). Protects proteins in vitro against tannin inactivation; tannins have antimicrobial activity. Overexpression enhances the stability of otherwise unstable periplasmic proteins.
  • 基因功能参考文献：
    1. Visualizing of Spy chaperone-assisted Im7 protein folding has been reported. PMID: 27239796
    2. Experimental results on Spy-Im7 interactions were used to construct a model for how this chaperone interacts with its clients. It appears that Spy binds to and releases client proteins in a regulated fashion that uses client protein folding as its own cue for release. PMID: 27293188
    3. Spy rapidly associates with its substrate, immunity protein 7 (Im7), thereby eliminating Im7's potential for aggregation. PMID: 26619265
    4. Establish a good relationship between the degree of instability and the improvement chaperone activity of spy variants. PMID: 24497545
    5. Report of the crystal structure of Escherichia coli spheroplast protein y (EcSpy). PMID: 20799348
    6. The induction of spy gene transcription by external zinc may include multiple steps such as through sensing the zinc-induced envelope disorder by BaeSR. PMID: 17884222

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  • 亚细胞定位：
    Periplasm.
  • 蛋白家族：
    CpxP/Spy family
  • 数据库链接：

    KEGG: ecj:JW1732

    STRING: 316385.ECDH10B_1881