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  4. RRP6 Antibody

RRP6 Antibody

  • 货号：
    CSB-PA619566XA01SVG
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in Recombinant protein
      All lanes: RRP6 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 54.7 kDa
      Observed band size: 58 kDa
  • 其他：

产品详情

  • Uniprot No.：
    Q12149
  • 基因名：
    RRP6
  • 别名：
    Exosome complex exonuclease RRP6 (EC 3.1.13.-) (Ribosomal RNA-processing protein 6) RRP6 UNC733 YOR001W
  • 反应种属：
    Bacillus thuringiensis subsp. Kurstaki
  • 免疫原：
    Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RRP6 protein
  • 免疫原种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Protein G
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Nuclear-specific catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and cryptic unstable transcripts (CUTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. RRP6 has 3'-5' exonuclease activity which is not modulated upon association with Exo-9 suggesting that the complex inner RNA-binding path is not used to access its active site.
  • 基因功能参考文献：
    1. Rrp41 mutations blocked substrate passage through the channel to Rrp44 only for cytoplasmic mRNAs, supporting the predicted widening of the lumen in the Rrp6-associated.Many exosome substrates exhibited clear preferences for a specific path to Rrp44. Both threading and direct access routes involve the RNA helicase Mtr4Rrp41 mutations blocked substrate passage through the channel to Rrp44 only for cytoplasmic mRNAs, suppor PMID: 28355211
    2. Rrp6: Integrated roles in nuclear RNA metabolism and transcription termination PMID: 26612606
    3. The data raise the intriguing possibility that Nab3 and Nrd1 can function independently to recruit Rrp6 to ncRNA targets, providing combinatorial flexibility in RNA processing. PMID: 25775092
    4. The exosome-binding factors Rrp6 and Rrp47 form a composite surface for recruiting the Mtr4 helicase. PMID: 25319414
    5. Rrp6 acts with the NNS complex(Nrd1, Nab3, and Sen1 ) globally to promote transcription termination in addition to 3' end RNA processing and/or degradation at specific targets. PMID: 25680078
    6. 3.3 A crystal structure of a ten-subunit RNA exosome complex from Saccharomyces cerevisiae composed of the Exo9 core and Rrp6 bound to single-stranded poly(A) RNA PMID: 25043052
    7. Rrp47p reduces the exonucleolytic activity of Rrp6p on both single-stranded and structured RNA substrates without significantly altering the affinity towards RNA or the ability of Rrp6p to degrade RNA secondary structure. PMID: 24937447
    8. results demonstrate that a principal function of Rrp47 is to facilitate appropriate expression levels of Rrp6. PMID: 24224060
    9. In the nucleus of yeast cells, an eleventh component Rrp6 binds to the exosome, providing a second exoribonucleolytic site to the entire complex. PMID: 24189234
    10. Rrp47 and Rrp6 have resolvable functions in Rrp6-mediated RNA surveillance and processing pathways. PMID: 24106327
    11. Assembly of the yeast exoribonuclease Rrp6 with its associated cofactor Rrp47 occurs in the nucleus and is critical for the controlled expression of Rrp47 PMID: 23580640
    12. Rrp6 plays a role in degrading distinct classes of meiotically induced noncoding RNAs during vegetative growth and the onset of meiosis. PMID: 21149693
    13. Rrp6 is required for repair of UV-induced DNA damage PMID: 15489286
    14. the major function of Rrp6p is in RNA surveillance PMID: 16260613
    15. transcripts are somehow distinguished from true mRNAs and are degraded in an Rrp6p-dependent fashion in the nucleus PMID: 16484372
    16. Nab6p binds poly(A)+ RNA, and the functions of Nab6p and Los1p suggest that mRNA metabolism and/or protein synthesis are growth rate limiting in rrp6Delta strains. PMID: 17101774
    17. Results identify a domain within the yeast nucleolus that is enriched in polyadenylated RNAs in the absence of the nuclear exosome RNase Rrp6 or the exosome cofactor Mtr4. PMID: 17403903
    18. Results suggest Rrp47p promotes Rrp6p activity by facilitating binding via the PMC2NT domain to structural elements within RNA. PMID: 17704127
    19. The 3'-5' exonucleolytic domain of Rrp6p is necessary for establishing all quality control phenotypes associated with THO mutations. PMID: 18824516

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  • 亚细胞定位：
    Nucleus, nucleolus.
  • 蛋白家族：
    Exosome component 10/RRP6 family
  • 数据库链接：

    KEGG: sce:YOR001W

    STRING: 4932.YOR001W