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  4. Phospho-CCDC88A (Ser1417) Antibody

Phospho-CCDC88A (Ser1417) Antibody

  • 货号：
    CSB-PA550459
  • 规格：
    ￥2454
  • 图片：
    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Girdin (Phospho-Ser1417) antibody (left)or the same antibody preincubated with blocking peptide (right).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) CCDC88A Polyclonal antibody
  • Uniprot No.：
    Q3V6T2
  • 基因名：
    CCDC88A
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse
  • 免疫原：
    Peptide sequence around phosphorylation site of Serine 417(L-E-M(p)-A-Q) derived from Human Girdin .
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Bifunctional modulator of guanine nucleotide-binding proteins (G proteins). Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates guanine nucleotide-binding protein G(i) alpha subunits. Also acts as a guanine nucleotide dissociation inhibitor for guanine nucleotide-binding protein G(s) subunit alpha GNAS. Essential for cell migration. Interacts in complex with G(i) alpha subunits with the EGFR receptor, retaining EGFR at the cell membrane following ligand stimulation and promoting EGFR signaling which triggers cell migration. Binding to Gi-alpha subunits displaces the beta and gamma subunits from the heterotrimeric G-protein complex which enhances phosphoinositide 3-kinase (PI3K)-dependent phosphorylation and kinase activity of AKT1/PKB. Phosphorylation of AKT1/PKB induces the phosphorylation of downstream effectors GSK3 and FOXO1/FKHR, and regulates DNA replication and cell proliferation. Binds in its tyrosine-phosphorylated form to the phosphatidylinositol 3-kinase (PI3K) regulatory subunit PIK3R1 which enables recruitment of PIK3R1 to the EGFR receptor, enhancing PI3K activity and cell migration. Plays a role as a key modulator of the AKT-mTOR signaling pathway, controlling the tempo of the process of newborn neuron integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Inhibition of G(s) subunit alpha GNAS leads to reduced cellular levels of cAMP and suppression of cell proliferation. Essential for the integrity of the actin cytoskeleton. Required for formation of actin stress fibers and lamellipodia. May be involved in membrane sorting in the early endosome. Plays a role in ciliogenesis and cilium morphology and positioning and this may partly be through regulation of the localization of scaffolding protein CROCC/Rootletin.
  • 基因功能参考文献：
    1. Results showed a role of Girdin in the collective invasion of skin cancer cells, where it interacts with beta-catenin. Also, Girdin is indispensable for stable cell-cell interaction, supracellular cytoskeletal organization, and the collective migration of cancer. PMID: 30194792
    2. Study revealed that the downregulation of the expression of Girdin can inhibit the proliferation, invasion and migration of colorectal cancer cells through decrease in proinflammatory cytokine production and inhibition of JAK/STAT signaling. PMID: 29989653
    3. Girdin may regulate cell processes. PMID: 29901184
    4. The engulfment of platelets assisted in delaying the aging of endothelial cells via girdin and pgirdin, in which the AKT signal was involved. PMID: 29786109
    5. Girdin can regulate glycolysis in hepatocellular carcinoma cells through the PI3K/AKT/HIF-1alpha signaling pathway, which decreases the sensitivity of tumor cells to radiotherapy. PMID: 28810896
    6. Girdin expression may serve as a useful prognostic factor for invasive breast cancer, especially for the HER2 subtype. PMID: 28818465
    7. The present study therefore suggests a role for Girdin as a novel therapeutic target for breast cancer, independent of subtype. PMID: 28713924
    8. GIV is a bifunctional modulator of G proteins; it serves as a guanine nucleotide dissociation inhibitor (GDI) for Galphas using the same motif that allows it to serve as a guanine-nucleotide exchange factor for Galphai PMID: 27621449
    9. GIV (Girdin) expression status predicts recurrence risk in patients with T3 pMMR stage II colon cancer. PMID: 27029492
    10. Results show that high CCDC88A expression in human pancreatic ductal adenocarcinoma (PDAC) tissues is correlated with poor prognosis. Also, the findings suggest that CCDC88A can promote PDAC cell migration and invasion through a signaling pathway that involves phosphorylation/dephosphorylation of many proteins. PMID: 27919290
    11. Here, thes identify GIV/Girdin as a novel effector of AMPK, whose phosphorylation at a single site is both necessary and sufficient for strengthening mammalian epithelial tight junctions and preserving cell polarity and barrier function in the face of energetic stress. PMID: 27813479
    12. On the basis of the differential prognostic impact of tGIV/pYGIV within each molecular subtype, we propose a diagnostic algorithm PMID: 27440794
    13. Results show that Girdin is important for formation and function of invadopodia enhanced by Dlg5-silencing in hepatocellular carcinoma cells. PMID: 28390157
    14. Tyrosine Phosphorylation of an Actin-Binding Protein Girdin Specifically Marks Tuft Cells in Human and Mouse Gut PMID: 28375676
    15. Overexpression of girdin is associated with invasion of hepatocellular carcinoma. PMID: 27623945
    16. Heterotrimeric G protein signaling via GIV/Girdin is a ubiquitous mechanism in health and disease, and can be a target for molecular therapies. (Review) PMID: 26879989
    17. miR-101 inhibits cell proliferation, migration and invasion in hepatocellular carcinoma through downregulating Girdin. PMID: 26743900
    18. Phosphorylation of GIV at Tyr-1764/Tyr-1798 is also required to enhance PI3K-Akt signaling and tumor cell migration in response to integrin stimulation, indicating that GIV functions in Tyr(P)-dependent integrin signaling. PMID: 26887938
    19. CCDC88A is essential for multiple aspects of normal development and loss of CCDC88A is a cause of the PEHO syndrome phenotype. PMID: 26917597
    20. GIV is an essential upstream component that couples InsR to G-protein signaling to enhance the metabolic insulin response, and impairment of such coupling triggers IR. PMID: 26378251
    21. Girdin regulates the migration and invasion of glioma cells via the PI3K-Akt signaling pathway. PMID: 26151295
    22. The expression of Girdin protein in invasive breast cancer is strongly associated with lymph node metastasis. PMID: 24155038
    23. GIV expression is up-regulated in liver after fibrotic injury and is required for hepatic stellate cells activation.Girdin is a central hub for profibrogenic signalling networks during liver fibrosis. PMID: 25043713
    24. TAT-GIV peptides provide a novel and versatile tool to manipulate Galphai activation downstream of growth factors in a diverse array of pathophysiologic conditions. PMID: 25926659
    25. transcriptional upregulation of Girdin expression and Girdin-Galphai3 signaling play crucial roles in regulating epithelial apicobasal polarity through the PAR complex. PMID: 25977476
    26. GIV directly and constitutively binds the exocyst complex subunit Exo-70 and also associates with GLUT4-storage vesicles (GSVs) exclusively upon insulin stimulation. PMID: 26514725
    27. Expression of tumor necrosis factor receptor-assicated factor 4 correlates with expression of Girdin and promotes nuclear translocation of Girdin in breast cancer PMID: 25591657
    28. GIV and its substrate Galphai3 are recruited to active integrin complexes PMID: 26391662
    29. the positive expression rate of Girdin in hepatocellular carcinoma tissues was 67.5%, higher than that found in adjacent tissues of 16.7% PMID: 25755745
    30. findings suggest that the STAT3/Girdin/Akt pathway activates in osteoblasts in response to mechanical stimulation and may play a significant role in triggering osteoblast proliferation and migration during orthodontic treatment PMID: 26163263
    31. Girdin regulates the trafficking of VE-cadherin in synergy with R-Ras. PMID: 25869066
    32. Both SH2 and GEF domains of GIV are required for the formation of a ligand-activated ternary complex between GIV, Galphai3, and EGFR. PMID: 25187647
    33. The study shows that girdin is phosphorylated on tyrosine 1798 when associated with structures required for migration. PMID: 25707853
    34. The review discusses how GIV assembles alternative signaling pathways by sensing cues from various classes of surface receptors and relaying them via G protein activation. The dysregulation of this mechanism in disease is discussed. [review] PMID: 25605737
    35. Findings demonstrate that Dlg5 interacts with and inhibits the activity of Girdin, thereby suppressing the migration of prostate cancer cells. PMID: 24662825
    36. Girdin knockdown enhances chemosensitivity of colorectal cancer cells to oxaliplatin via TOP2B down-regulation. PMID: 25009397
    37. This study showed that reduction of Girdin, an actin-binding protein, leads to impaired cell migration, adhesion, and invasion of human glioblastoma cells. PMID: 25060559
    38. These results reveal that girdin regulates selective clathrin-mediated endocytosis via a mechanism involving dynamin 2, but not by operating as a cargo-specific adaptor. PMID: 25061227
    39. This study identified a novel GWS association (1.17 x 10(-10)) mapped to chromosome 2 at rs1437396, between MTIF2 and CCDC88A, across all of the EA and AA cohorts. PMID: 24166409
    40. Girdin was identified as a new and major regulator of the insulin signal in myoblasts and skeletal muscle. PMID: 23886629
    41. Up-regulated autophagy was negatively associated with Girdin level. There was a significant correlation between Girdin expression and lymph nodes metastasis in invasive ductal breast carcinoma. PMID: 24326843
    42. The levels of Girdin expression correlated inversely with the survival of esophageal squamous cell carcinoma patients. PMID: 23588413
    43. These results demonstrate that girdin and its phosphorylation play an important role in neonatal vascular development and in pathological neovascularization in the retina. PMID: 23195430
    44. Girdin protein may be a potential new distant metastasis biomarker of breast cancer PMID: 22116776
    45. Our findings define EEA1 endosomes as major sites for proliferative signaling and establish that Galphas and GIV regulate EEA1 but not APPL endosome maturation PMID: 23051738
    46. p-Girdin expression is closely correlated with the malignant progression of breast cancer. PMID: 22780975
    47. STAT3 activation is directly integrated with the receptor tyrosine kinase-GIV-G protein signaling axis. PMID: 23066027
    48. The Girdin protein may be a potential new early liver metastasis biomarker of colorectal cancer. PMID: 22714912
    49. These data demonstrate that Girdin is important for efficient cell division PMID: 22755556
    50. Girdin regulates cell movement in biological contexts that require directional cell movement PMID: 22574214

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  • 相关疾病：
    PEHO-like syndrome (PEHOL)
  • 亚细胞定位：
    Cell membrane; Peripheral membrane protein. Cytoplasm, cytosol. Cytoplasmic vesicle. Cell projection, lamellipodium. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole.
  • 蛋白家族：
    CCDC88 family
  • 组织特异性：
    Expressed ubiquitously.
  • 数据库链接：

    HGNC: 25523

    OMIM: 609736

    KEGG: hsa:55704

    STRING: 9606.ENSP00000338728

    UniGene: Hs.292925