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GNAI3 Antibody

  • 货号：
    CSB-PA576871
  • 规格：
    ￥1100
  • 图片：
    • The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using CSB-PA576871(GNAI3 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
  • 其他：

产品详情

  • Uniprot No.：
    P08754
  • 基因名：
  • 别名：
    87U6 antibody; FLJ26559 antibody; G protein alpha inhibiting 3 antibody; G(i) alpha 3 antibody; G(i) alpha-3 antibody; GNAI3 antibody; GNAI3_HUMAN antibody; Guanine nucleotide binding protein (G protein) alpha inhibiting activity polypeptide 3 antibody; Guanine nucleotide binding protein G(k) alpha subunit antibody; Guanine nucleotide-binding protein G(k) subunit alpha antibody; OTTHUMP00000013368 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Fusion protein of Human GNAI3
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen affinity purification
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,IHC
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:1000-1:2000
    IHC 1:25-1:100
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins. Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels. Stimulates the activity of receptor-regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.
  • 基因功能参考文献：
    1. magnetic field-dependent nuclear magnetic resonance relaxation analyses were used to investigate the structural and dynamic properties of GDP bound Galpha on a microsecond timescale. PMID: 28223697
    2. Results show that Galphai3 nuclear translocation causes irradiation resistance in human glioma cells through its complexation with DNA-PKcs leading to DNA repair. PMID: 28456783
    3. GNAI3 is identified a second gene possibly responsible for X-linked ocular albinism. PMID: 27607449
    4. Data show that auriculo-condylar syndrome (ACS)-associated mutations in G protein subunit alpha i3 (GNAI3) produce dominant-negative Galpha(i3) mutant proteins that couple to endothelin type A receptor (ET(A)R). PMID: 27072656
    5. In postmortem human prefrontal cortex, adenosine A1 receptor is coupled preferentially, if not exclusively, to Galphai-3. PMID: 26213104
    6. transcriptional upregulation of Girdin expression and Girdin-Galphai3 signaling play crucial roles in regulating epithelial apicobasal polarity through the PAR complex. PMID: 25977476
    7. GIV and its substrate Galphai3 are recruited to active integrin complexes PMID: 26391662
    8. We demonstrate that the GNAI3 variant is the likely cause of auriculocondylar syndrome in the original ACS1 family. PMID: 25026904
    9. Both SH2 and GEF domains of GIV are required for the formation of a ligand-activated ternary complex between GIV, Galphai3, and EGFR. PMID: 25187647
    10. Low GNAI3 expression is associated with hepatocellular carcinoma. PMID: 25444921
    11. Data indicate that dynein- and astral microtubule-mediated transport of Galphai/LGN/nuclear mitotic apparatus (NuMA) complex from cell cortex to spindle poles. PMID: 23389635
    12. The phenotypic variability of auriculocondylar syndrome suggests that mutations in this pathway, especially those affecting core signaling molecules such as PLCB4 and GNAI3, should be considered as potential candidates for other ear and jaw malformations. PMID: 22560091
    13. These results identify the Oa1 transducer Galphai3 as the first downstream component in the Oa1 signaling pathway. PMID: 21931697
    14. The mechanisms of regulation of GIRK by Galpha(i/o) using wild-type Galpha(i3) (Galpha(i3)WT) and Galpha(i3), were investigated. PMID: 21795707
    15. These results provide mechanistic insights into how reversible modulation of Galpha(i3) activity by AGS3 and GIV maintains the delicate equilibrium between promotion and inhibition of autophagy. PMID: 21209316
    16. Data suggest that Galphai-TNFAIP8-mediated rescue of pre-oncogenic cells enhances progression to oncogenic transformation, providing a selective target to inhibit cellular transformation. PMID: 20607800
    17. we show that A3 adenosine receptor/Gi3 play important roles in human mast cells responses initiated on contact with activated T cells. PMID: 20190146
    18. These data present AGS3, G-proteins, and mInsc as candidate proteins involved in regulating cellular stress associated with protein-processing pathologies. PMID: 20065032
    19. Overexpression of GIPN stimulates proteasome-dependent reduction of endogenous G alpha i3 in HEK293 cells and reduces the half-life of overexpressed G alpha i3-YFP. PMID: 12826607
    20. diffraction data were collected to 2.5 A resolution at 100 K using synchrotron radiation at Pohang beamline 4A for human RGS10 complexed with Galphai3 [Galphai3] PMID: 16511171
    21. x-ray crystallography of the complex of RGS5 and Galphai(3) proteins with GDP/Mg(2+)/AlF(4)(-) at 3.0 A resolution PMID: 17100651
    22. The Galphai3-GIV switch serves to link direction sensing from different families of chemotactic receptors to formation of the leading edge during cell migration. PMID: 18663145
    23. Insulin-like growth factor-binding protein-5 stimulates growth of human intestinal muscle cells by activation of Galphai3. PMID: 19808657

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  • 相关疾病：
    Auriculocondylar syndrome 1 (ARCND1)
  • 亚细胞定位：
    Cytoplasm. Cell membrane; Lipid-anchor. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
  • 蛋白家族：
    G-alpha family, G(i/o/t/z) subfamily
  • 数据库链接：

    HGNC: 4387

    OMIM: 139370

    KEGG: hsa:2773

    STRING: 9606.ENSP00000358867

    UniGene: Hs.73799