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GFI1B Antibody

  • 货号：
    CSB-PA257884
  • 规格：
    ￥2024
  • 图片：
    • Western blot analysis of extracts from Raji cells using GFI1b Antibody.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) GFI1B Polyclonal antibody
  • Uniprot No.：
    Q5VTD9
  • 基因名：
    GFI1B
  • 宿主：
    Rabbit
  • 反应种属：
    Human,Mouse,Rat
  • 免疫原：
    Peptide sequence around aa.322~326(H-R-E-S-Q) derived from Human GFI1b.
  • 免疫原种属：
    Homo sapiens (Human)
  • 克隆类型：
    Polyclonal
  • 纯化方式：
    Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA,WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:1000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Component of a RCOR-GFI-KDM1A-HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. Transcriptional repressor or activator depending on both promoter and cell type context; represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1; GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down-regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription; as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma-satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation.
  • 基因功能参考文献：
    1. Disruption of GFI1B non-DNA-binding zinc-finger 1 is associated with mild to moderate thrombocytopenia without alpha-granule deficiency or bleeding symptomatology, indicating that the site of GFI1B mutation has important phenotypic implications. Platelet CD34 expression appears to be a common feature of perturbed GFI1B function. PMID: 28880435
    2. Platelet CD34 expression and alpha/delta-granule abnormalities in GFI1B- and RUNX1-related familial bleeding disorders. PMID: 28096094
    3. GFI1B is an essential protein for the normal development of the megakaryocyte lineage PMID: 27122003
    4. High GFI1B expression is associated with small-cell lung cancer. PMID: 28667074
    5. Results demonstrate that alpha-delta platelet storage pool deficiency is associated with either a heterozygous mutation in GFI1B (de novo or familial) abrogating the binding of the zinc fingers with the promoter of its target genes, or by hypomorphic biallelic mutations leading to autosomal recessive inheritance. PMID: 28041820
    6. study demonstrates the significance of Gfi1b regulated Kindlin3-Talin1 expression in driving megakaryocytic differentiation and highlights the contribution of cytoskeletal agents in the developmental progression of these platelet progenitors PMID: 27768697
    7. Role for Alternative GFI1B Splice Variants in Human Hematopoiesis PMID: 27486782
    8. SPI1-GFI1B transcriptional network is an important regulatory axis in acute myeloid leukemia as well as in the development of erythroid versus myelomonocytic cell fate PMID: 26851695
    9. Gfi1b functions as a transcriptional repressor by recruiting histone-modifying enzymes to promoters and enhancers of target genes. Mutations are associated with certain bleeding disorders. Review. PMID: 26447191
    10. a mutation in GFI1B causes a platelet disorder; GFI1B has a role a critical regulator of platelet shape, number, and function PMID: 23927492
    11. results, supported by evidence from mouse models, identify GFI1 and GFI1B as prominent medulloblastoma oncogenes and implicate 'enhancer hijacking' as an efficient mechanism driving oncogene activation in a childhood cancer PMID: 25043047
    12. TAL1 is involved in regulating H3K27me3 variations in collaboration with GFI1B PMID: 24395799
    13. Silencing of both BCR-ABL siRNA and GFI1B siRNA is associated with an additive antileukemic effect against K562 cells. PMID: 23788109
    14. Our studies show that GFI1B, in addition to being causally related to the gray platelet syndrome, is key to megakaryocyte and platelet development. PMID: 24325358
    15. Gfi-1B p32, a Gfi-1B isoform, is essential for erythroid differentiation PMID: 22399799
    16. GFI1B regulatory elements behave as activators and repressors, consistent with a model in which GFI1B binds to its own promoter and to the conserved non-coding elements as its levels rise. PMID: 19773260
    17. Results reveal the presence of different protein complexes, including GATA-1 and Oct-1, involved in Gfi1b regulation. PMID: 20143233
    18. repressing TGF-beta type III receptor (TbetaRIotaII) expression, Gfi-1B favors the Smad2/TIF1-gamma interaction downstream of TGF-beta signaling, allowing immature progenitors to differentiate toward the erythroid lineage. PMID: 20124515
    19. HMGB2 potentiates GATA-1-dependent transcription of GFI1B by Oct-1 and thereby controls erythroid differentiation. PMID: 19965638
    20. This zinc finger protein mediates erythroid expansion and has a role in normal erythropoiesis PMID: 12351384
    21. GATA-1 and NF-Y both contribute to erythroid-specific transcriptional activation of the Gfi-1B promoter. PMID: 15280509
    22. Gfi-1B acts in the late stage of erythroid differentiation as a transcriptional repressor. PMID: 15507521
    23. GATA-1 and Gfi-1B participates in a feedback regulatory pathway in controlling the expression of the Gfi-1B gene. PMID: 16177182
    24. E2A proteins prevent lymphoma cell expansion, at least in part through regulation of Gfi1b and modulation of Gata3 expression. PMID: 17272506
    25. GATA1 and GFI1B interplay to regulate bcl-X protein transcription. PMID: 17420275
    26. suggest that Gfi1b may be an important factor to establish or maintain myeloid leukemia and myeloproliferative diseases PMID: 19360458
    27. Data conclude that Gfi-1B behaves as a lineage-affiliated gene with an open chromatin configuration in multipotent progenitors and sustained activation as cells progress throughout erythroid differentiation. PMID: 19522008

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  • 相关疾病：
    Bleeding disorder, platelet-type 17 (BDPLT17)
  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Expressed in bone marrow and fetal liver, but also detectable in fetal spleen, fetal thymus, and testes. Detected in hematopoietic stem cells, erythroblasts, and megakaryocytes. Overexpressed in bone marrow of patients with erythroleukemia and megakaryocy
  • 数据库链接：

    HGNC: 4238

    OMIM: 187900

    KEGG: hsa:8328

    STRING: 9606.ENSP00000344782

    UniGene: Hs.553160