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DACT1 Antibody

  • 货号：
    CSB-PA873691LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA873691LA01HU diluted at 1:600 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA873691LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) DACT1 Polyclonal antibody
  • Uniprot No.：
    Q9NYF0
  • 基因名：
    DACT1
  • 别名：
    Dact antibody; dact1 antibody; DACT1_HUMAN antibody; Dapper antagonist of beta catenin homolog 1 antibody; Dapper antagonist of catenin 1 antibody; Dapper antibody; Dapper homolog 1 antibody; DAPPER1 antibody; Dpr antibody; Dpr1 antibody; Frd antibody; Frodo antibody; hDPR1 antibody; Hepatocellular carcinoma novel gene 3 protein antibody; Heptacellular carcinoma novel gene 3 antibody; HNG3 antibody; MTNG3 antibody; Thyex3 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Dapper homolog 1 protein (410-525AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，DACT1 Antibody (CSB-PA873691LA01HU)，的标记方式是Non-conjugated。对于DACT1 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA873691LB01HU DACT1 Antibody, HRP conjugated ELISA
    FITC CSB-PA873691LC01HU DACT1 Antibody, FITC conjugated
    Biotin CSB-PA873691LD01HU DACT1 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:500-1:1000
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Involved in regulation of intracellular signaling pathways during development. Specifically thought to play a role in canonical and/or non-canonical Wnt signaling pathways through interaction with DSH (Dishevelled) family proteins. The activation/inhibition of Wnt signaling may depend on the phosphorylation status. Proposed to regulate the degradation of CTNNB1/beta-catenin, thereby modulating the transcriptional activation of target genes of the Wnt signaling pathway. Its function in stabilizing CTNNB1 may involve inhibition of GSK3B activity. Promotes the membrane localization of CTNNB1. The cytoplasmic form can induce DVL2 degradation via a lysosome-dependent mechanism; the function is inhibited by PKA-induced binding to 14-3-3 proteins, such as YWHAB. Seems to be involved in morphogenesis at the primitive streak by regulating VANGL2 and DVL2; the function seems to be independent of canonical Wnt signaling and rather involves the non-canonical Wnt/planar cell polarity (PCP) pathway. The nuclear form may prevent the formation of LEF1:CTNNB1 complex and recruit HDAC1 to LEF1 at target gene promoters to repress transcription thus antagonizing Wnt signaling. May be involved in positive regulation of fat cell differentiation. During neuronal differentiation may be involved in excitatory synapse organization, and dendrite formation and establishment of spines.
  • 基因功能参考文献：
    1. DACT1alpha plays a pivotal role as a potential tumor suppressor in migration and invasion of gastric cancer. DACT1alpha methylation may serve as a biomarker for the prognosis of gastric cancer. PMID: 27833078
    2. Findings suggest that the DACT1 c.1256G>A nonsense variant is causative of a specific genetic syndrome with features overlapping Townes-Brocks syndrome. PMID: 28054444
    3. Dapper1 attenuates hepatic gluconeogenesis and lipogenesis in Ttype 2 diabetes. PMID: 28237722
    4. An inhibitory role for DACT1 in leukemogenesis. PMID: 29037126
    5. Dact1 is up-regulated by TGF-beta1, inducing apoptosis in mesangial cells. PMID: 27714812
    6. The simultaneous methylation of DACT1 and DACT2 may play important roles in progression of ESCC and may serve as prognostic methylation biomarkers for ESCC patients. PMID: 28077137
    7. Dact1 has a critical role in the ability support keratinocyte proliferation, by attenuating Wnt/beta catenin signaling. PMID: 26099026
    8. our results suggested that DACT1 was upregulated during human placenta development. PMID: 25424899
    9. There was no statistical difference between groups concerning DACT1 and DACT2 either in promoter hypermethylation or transcript levels. Age was associated with DACT2 promoter hypermethylation, especially over 56 years old. PMID: 25524937
    10. Dpr1 promotes the ubiquitination of Dvl2 by pVHL and mediates the protein aggregate-elicited autophagy initiation PMID: 25825496
    11. Overexpression of Dapper-1 allows the translocation of MIZ-1 from the nucleus to the cytoplasm. PMID: 25558878
    12. Dpr1 directly interacts with Beclin1 and Atg14L and enhances the Beclin1-Vps34 interaction and Vps34 activity. PMID: 24980960
    13. Data indicate that Sestd1 cooperates with Dact1 in Vangl2 regulation and in the planar cell polarity (PCP) pathway during mammalian embryonic development. PMID: 23696638
    14. five missense heterozygote mutations of the DACT1 gene are specifically identified in 167 stillborn or miscarried Han Chinese fetuses with neural tube defects. PMID: 22610794
    15. These findings provided insight into the role of DACT1 as a novel functional tumor suppressor in gastric cancer through inhibiting NF-kappaB signaling pathway. PMID: 23073659
    16. DACT1 stabilizes beta-catenin via DACT1-induced effects on GSK-3beta and directly interacts with beta-catenin proteins. PMID: 22470507
    17. As(2)O(3) induces demethylation of hdpr1 gene from abnormal hypermethylation status and activates its reexpression, thus suppressing the proliferation of Jurkat cells. PMID: 21176356
    18. Cytoplasmic HDPR1 protein expression was associated with tumor malignant progression via beta-catenin accumulation. PMID: 21525190
    19. 14-3-3beta interacts with human Dapper1, attenuating the ability of hDpr1 to promote Dishevelled (Dvl) degradation, thus enhancing Wnt signaling PMID: 21262972
    20. Knockdown of HDPR1 gene enhanced the invasive ability of lung cancer cells, which was dependent on p120ctn and independent of beta-catenin PMID: 20232357
    21. Downregulation of HDPR1 is common in hepatic cellular carcinomas, frequently involves hypermethylation of the promoter region. PMID: 15580286
    22. DACT1 antagonizes Wnt signaling by promoting DVL2 degradation. PMID: 16446366
    23. Dact1 regulates adipogenesis through coordinated effects on gene expression that selectively alter intracellular and paracrine/autocrine components of the Wnt/beta-catenin signaling pathway. PMID: 19073771

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  • 相关疾病：
    Neural tube defects (NTD); Townes-Brocks syndrome 2 (TBS2)
  • 亚细胞定位：
    Cytoplasm. Nucleus. Cell junction, synapse. Note=Shuttles between the nucleus and the cytoplasm. Seems to be nuclear in the absence of Wnt signaling and to translocate to the cytoplasm in its presence.
  • 蛋白家族：
    Dapper family
  • 数据库链接：

    HGNC: 17748

    OMIM: 182940

    KEGG: hsa:51339

    STRING: 9606.ENSP00000337439

    UniGene: Hs.48950