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CYTH2 Antibody

  • 货号：
    CSB-PA857445ESR1HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western blot
      All lanes: CYTH2 antibody at 5.58μg/ml
      Lane 1: U251 whole cell lysate
      Lane 2: HepG2 whole cell lysate
      Lane 3: MCF-7 whole cell lysate
      Lane 4: A549 whole cell lysate
      Secondary
      Goat polyclonal to rabbit IgG at 1/10000 dilution
      Predicted band size: 47 kDa
      Observed band size: 47, 35 kDa
    • Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA857445ESR1HU at dilution of 1:100
    • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA857445ESR1HU at dilution of 1:100
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) CYTH2 Polyclonal antibody
  • Uniprot No.：
    Q99418
  • 基因名：
  • 别名：
    ARF exchange factor antibody; ARF nucleotide binding site opener antibody; ARF nucleotide-binding site opener antibody; Arno antibody; ARNO protein antibody; CLM2 antibody; CTS18 antibody; CTS18.1 antibody; CYH2_HUMAN antibody; Cyth2 antibody; Cytohesin 2 antibody; Cytohesin-2 antibody; MGC137537 antibody; MGC80440 antibody; PH antibody; PH; SEC7 and coiled-coil domain-containing protein 2 antibody; Pleckstrin homology Sec7 and coiled coil domains 2 antibody; Pleckstrin homology Sec7 and coiled coil domains protein 2 antibody; Protein ARNO antibody; PSCD2 antibody; PSCD2L antibody; PSCD2L; formerly antibody; SEC7 and coiled-coil domain-containing protein 2 antibody; Sec7 antibody; SEC7 homolog B antibody; Sec7B antibody; SEC7L antibody; Sec7p L antibody; Sec7p-like antibody; Sec7pL antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Cytohesin-2 protein (150-350AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity Purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Activates ARF factors through replacement of GDP with GTP. The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane. Involved in neurite growth.
  • 基因功能参考文献：
    1. ARNO-ARF1 regulates formation of podosomes by inhibition of RhoA/myosin-II and promotion of actin core assembly. PMID: 28007915
    2. s' experiments argue against ARNO being a robust modifier of EGFR catalytic activity. PMID: 27203102
    3. Data establish a role for cytohesin-2/ARNO as a regulator of R-Ras and integrin recycling and suggest that ARF-regulated trafficking of R-Ras is required for R-Ras-dependent effects on spreading and adhesion formation. PMID: 26378252
    4. The s show that Shigella flexneri IpgD phosphatase activity is required for recruitment of the ARF6 guanine nucleotide exchange factor (GEF) ARF nucleotide binding site opener (ARNO) to bacterial entry sites. PMID: 25736891
    5. inhibiting cytohesins or ARNO as cytoplasmic activators of EGFR and IGF-I in colorectal cancer resulted in anti-proliferation, reduced invasion, decreased migration, and suppressed growth in vivo and in vitro. PMID: 24618737
    6. Cytohesin-2 constitutively suppresses platelet dense granule secretion and aggregation by keeping ARF6 in a GTP-bound state PMID: 24581425
    7. Expression of CYTH2 mutant deficient of the EFh2 domain in cells also inhibits Arf6 activation and neurite extension. PMID: 22659138
    8. Phosphorylation of Ser392 of ARNO stabilized the C-terminal alpha-helix via formation of salt bridges between phospho-Ser392 and Arg390, Lys395, and Lys396. PMID: 24083777
    9. There is an association between cytohesin-2 expression and overall survival and disease-free survival in patients with hepatocellular carcinoma. PMID: 23545718
    10. Arf6/ ARNO signaling mediates phospholipase-D, ERK1/2 and cofilin activation in pancreatic beta-cells. PMID: 23095975
    11. Kinetics of interaction between ADP-ribosylation factor-1 (Arf1) and the Sec7 domain of Arno guanine nucleotide exchange factor, modulation by allosteric factors, and the uncompetitive inhibitor brefeldin A PMID: 23255605
    12. The N termini of a-subunit isoforms are involved in signaling between vacuolar H+-ATPase (V-ATPase) and cytohesin-2 PMID: 23288846
    13. ARNO in turn triggered WAVE regulatory complex recruitment and activation, which was dramatically enhanced when ARNO cooperated with Salmonella SopE. PMID: 22341462
    14. role for endothelial ARNO in VEGF-dependent initiation of angiogenesis by regulation of VEGFR-2 internalization in endothelial cells, resulting in the activation of the Akt pathway, vessel permeability, and ultimately endothelial proliferation PMID: 22002459
    15. Data that aldolase forms a complex with ARNO/Arf6 and the V-ATPase and that it may contribute to remodeling of the actin cytoskeleton. PMID: 21307348
    16. Arno behaves as a bistable switch, having an absolute requirement for activation by an Arf protein but, once triggered, becoming highly active through the positive feedback effect of Arf1-GTP. PMID: 21118813
    17. cytohesin-2, through a previously unexplored complex formation with paxillin, regulates preadipocyte migration; paxillin plays a previously unknown role as a scaffold protein of Arf guanine-nucleotide exchange factor PMID: 20525696
    18. Specific motifs of the V-ATPase a2-subunit isoform interact with catalytic and regulatory domains of ARNO. PMID: 20153292
    19. protein-protein interaction mediated by ARNO coiled-coil domain required for ARNO induced motility; coiled-coil domain promotes assembly of multiprotein complex containing ARNO and Dock180; assembly of complex requires coiled-coil domain, GRASP and IPCEF PMID: 20016009
    20. The overexpression of ARNO, another mammalian GEF, produces extensive neuritogenesis in Aplysia neurons PMID: 12641750
    21. cytohesin 2 binds to IPCEF1, which modifies its activity PMID: 12920129
    22. the N-terminal coiled-coil and parts of the Sec7 domain of cytohesin-2 are required for serum-mediated transcriptional activation in nonimmune cells PMID: 15277685
    23. Endogenous levels of ARNO/cytohesin-2 present in HEK293 cells are sufficient and necessary for sustained activation of the MAP kinase signaling pathway PMID: 16027149
    24. The transport and regulation of ARNO in polartized epithelial cells, and its interactions with ARF6 in endocytosis are reported. PMID: 16484220
    25. Study shows that three related Arf-like GTPases Arl4a, Arl4c, and Arl4d, are able to recruit ARNO and other cytohesins to the plasma membrane by binding to their PH domains irrespective of whether they are in the diglycine or triglycine form. PMID: 17398095
    26. data suggest cells ruffle upon CaSR (calcium sensing receptor)stimulation via a mechanism that involves translocation of beta-arrestin 1 pre-assembled with the CaSR or ARNO (Arf nucleotide binding site opener) PMID: 17623778

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  • 亚细胞定位：
    Cell membrane; Peripheral membrane protein. Cytoplasm. Cell projection. Cell projection, growth cone. Cell junction, tight junction. Cell junction, adherens junction.
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    HGNC: 9502

    OMIM: 602488

    KEGG: hsa:9266

    STRING: 9606.ENSP00000408236

    UniGene: Hs.144011