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CCNF Antibody

  • 货号：
    CSB-PA001882
  • 规格：
    ￥880
  • 图片：
    • Western Blot analysis of HuvEc cells using Cyclin F Polyclonal Antibody
  • 其他：

产品详情

  • Uniprot No.：
    P41002
  • 基因名：
    CCNF
  • 别名：
    Ccnf antibody; CCNF_HUMAN antibody; Cyclin-F antibody; F box only protein 1 antibody; F-box only protein 1 antibody; FBX1 antibody; FBXO1 antibody; G2 mitotic specific cyclin F antibody; G2/mitotic specific cyclin F antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Synthesized peptide derived from the C-terminal region of Human Cyclin F.
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 纯化方式：
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    WB, IHC, ELISA
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:100-1:300
    ELISA 1:10000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. The SCF(CCNF) E3 ubiquitin-protein ligase complex is an integral component of the ubiquitin proteasome system (UPS) and links proteasome degradation to the cell cycle. Mediates the substrate recognition and the proteasomal degradation of various target proteins involved in the regulation of cell cycle progression and in the maintenance of genome stability. Mediates the ubiquitination and proteasomal degradation of CP110 during G2 phase, thereby acting as an inhibitor of centrosome reduplication. In G2, mediates the ubiquitination and subsequent degradation of ribonucleotide reductase RRM2, thereby maintaining a balanced pool of dNTPs and genome integrity. In G2, mediates the ubiquitination and proteasomal degradation of CDC6, thereby suppressing DNA re-replication and preventing genome instability. Involved in the ubiquitination and degradation of the substrate adapter CDH1 of the anaphase-promoting complex (APC/C), thereby acting as an antagonist of APC/C in regulating G1 progression and S phase entry. May play a role in the G2 cell cycle checkpoint control after DNA damage, possibly by promoting the ubiquitination of MYBL2/BMYB.
  • 基因功能参考文献：
    1. A single missense mutation in cyclin F causes hyper-ubiquitylation of proteins that can indirectly impair the autophagy degradation pathway, which is implicated in amyotrophic lateral sclerosis pathogenesis. PMID: 28852778
    2. Cyclin F and ribonucleotide reductase family member 2 (RRM2) compose a functional axis responsible for nucleotide metabolism. Impairment in this pathway may contribute to increased DNA damage repair and drug resistance. Additionally, we analyzed the expression of RRM2 mRNA and discovered that high expression of RRM2 is associated with worse overall survival. PMID: 29767233
    3. Mutational spectrum of CCNF emphasizes the pathogenic role of CCNF mutations in ALS. PMID: 29102476
    4. Our result indicates that the mutation of CCNF is rare in patients with amyotrophic lateral sclerosis and frontotemporal dementia from Mainland China PMID: 28281833
    5. These data indicate that the coordinated, temporal ordering of cyclin F and Cdh1 degradation, organized in a double-negative feedback loop, represents a fundamental aspect of cell-cycle control PMID: 27653696
    6. s demonstrated a significant correlation between the severity of the CCNF-induced axonopathy and a reduced motor response to a light stimulus (photomotor response). PMID: 28444311
    7. Cyclin F-mediated degradation of SLBP limits H2A.X accumulation and apoptosis upon genotoxic stress in G2 cell cycle checkpoint. PMID: 27773672
    8. cyclin F is a novel F-box protein that functions as an intrinsic cellular regulator of HIV-1 Vif and has a negative regulatory effect on the maintenance of viral infectivity by restoring APOBEC3G expression PMID: 28184007
    9. The SCYL1- BP1 affects the cell cycle through increasing steady state levels of Cyclin F and RRM2 proteins, thus constituting a dual regulatory circuit. PMID: 25980818
    10. Data indicate that a missense mutation in cyclin F (CCNF) in a large Amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) family. PMID: 27080313
    11. CDC6 and Cyclin F interact through defined sequence motifs that promote CDC6 ubiquitylation and degradation. PMID: 26818844
    12. Cyclin F suppresses B-Myb activity to promote cell cycle checkpoint control. PMID: 25557911
    13. Cyclin F controls genome stability through ubiquitin-mediated proteolysis. (Review) PMID: 23182110
    14. After DNA damage, cyclin F is downregulated in an ATR-dependent manner to allow accumulation of RRM2. Defective elimination of cyclin F delays DNA repair and sensitizes cells to DNA damage, a phenotype that is reverted by expressing a nondegradable RRM2 mutant. PMID: 22632967
    15. SCF(Cyclin F)-mediated degradation of CP110 is required for the fidelity of mitosis and genome integrity PMID: 20596027
    16. degradation during G2-M by mechanisms fundamentally different from other cyclins PMID: 12122006

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  • 亚细胞定位：
    Nucleus. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole.
  • 蛋白家族：
    Cyclin family, Cyclin AB subfamily
  • 组织特异性：
    Widely expressed, with expression detected in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • 数据库链接：

    HGNC: 1591

    OMIM: 600227

    KEGG: hsa:899

    STRING: 9606.ENSP00000380256

    UniGene: Hs.1973