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KRT15 Monoclonal Antibody

  • 货号：
    CSB-MA785224
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-MA785224 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-MA785224 diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    Immunization of mice with a synthesized peptide derived from the human KRT15 protein is the first step in the production of the KRT15 monoclonal antibody. B cells isolated from the mouse spleen are fused with myeloma cells to generate hybridomas. Hybridomas are screened to identify those that produce KRT15 antibodies. The chosen hybridomas are cultured in the mouse abdominal cavity, and the KRT15 monoclonal antibodies are purified using affinity chromatography with a specific immunogen. The resulting KRT15 monoclonal antibody is a mouse monoclonal antibody that binds specifically to human KRT15 protein and can be used for ELISA and IHC applications.

    KRT15 is a type I intermediate filament protein that is mainly expressed in basal cells of the skin and other stratified epithelia. Its main function is to provide structural support to cells and tissues, and it plays a critical role in maintaining the integrity and strength of the skin. In addition, KRT15 has been implicated in the regulation of cell proliferation, differentiation, and migration, as well as wound healing and tissue repair processes. Mutations in the KRT15 gene have been linked to various skin disorders, including epidermolysis bullosa simplex and other keratinopathies.

  • 产品名称：
    Mouse anti-Homo sapiens (Human) KRT15 Monoclonal antibody
  • Uniprot No.：
    P19012
  • 基因名：
  • 别名：
    AI528832 antibody; CK 15 antibody; CK-15 antibody; CK15 antibody; Cytokeratin-15 antibody; Cytokeratin15 antibody; K15 antibody; K1C15_HUMAN antibody; K1CO antibody; Ka15 antibody; Keratin 15 antibody; Keratin 15 basic antibody; Keratin 15 beta antibody; Keratin antibody; Keratin complex 1 acidic gene 15 antibody; Keratin type I cytoskeletal 15 antibody; Keratin-15 antibody; Keratin15 antibody; KRT 15 antibody; Krt1-15 antibody; KRT15 antibody; KRTB antibody; KRTL15 antibody; Type I cytoskeletal 15 antibody; Type I keratin Ka15 antibody
  • 宿主：
    Mouse
  • 反应种属：
    Human
  • 免疫原：
    Synthesized peptide derived from human Cytokeratin 15
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    IgG1, Kappa
  • 纯化方式：
    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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