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  4. Sheep Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

Sheep Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

  • 中文名称：
    绵羊催乳素(PRL/LTH)酶联免疫试剂盒
  • 货号：
    CSB-E13161Sh
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Sheep PRL ELISA Kit was designed for the quantitative measurement of Sheep PRL protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 5 μIU/mL-2000 μIU/mL and the sensitivity is 5 μIU/mL.

  • 别名：
    PRL ELISA Kit; Prolactin ELISA Kit; PRL ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Ovis aries (Sheep)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    5 μIU/mL-2000 μIU/mL
  • 灵敏度：
    5 μIU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of sheep PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 88-99  
    1:2 Average % 94  
    Range % 84-101  
    1:4 Average % 93  
    Range % 85-103  
    1:8 Average % 90  
    Range % 87-101  
  • 回收率：
    The recovery of sheep PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 85-104  
    EDTA plasma (n=4) 95 88-99  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
                 
                 
                 
                 
                 
                 
                 
                 
                 
                 
                 
                 
                 
    μIU/ml OD1 OD2 Average    
    2000 0.154 0.149 0.152    
    375 0.338 0.351 0.345    
    62.5 0.542 0.572 0.557    
    17.5 0.983 0.959 0.971    
    5 1.515 1.529 1.522    
    0 2.164 2.077 2.121    
                 
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Prolactin acts primarily on the mammary gland by promoting lactation, mammogenesis, mitogenesis and osmoregulation.
  • 基因功能参考文献：
    1. These results indicate that PRL, EGF and glucocorticoids have distinct roles in the establishment of beta-casein secretion pathway. PMID: 27836773
    2. Prolactin induces apoptosis of lactotropes PMID: 24859278
    3. Prolactin and epidermal growth factor stimulate adipophilin synthesis in HC11 mouse mammary epithelial cells via the PI3-kinase/Akt/mTOR pathway. PMID: 22426621
    4. Differences in accessibility of the rat arcuate nucleus to sheep prolactin, together with intrinsic differences in neuroendocrine dopaminergic neurons, facilitate homeostatic feedback regulation of sheep prolactin release in the rat hypothalamus PMID: 21953590
    5. conclude that PRL is an important part of the regulation of the pituitary-gonadal system in moderately seasonal DLS rams; it seems to impair release of LH from gonadotrophs, help maintain functional integrity of Leydig cells, & promote testicular growth PMID: 19755483
    6. increased PRL concentration within the central nervous system (CNS) for a few days before oncoming ovulation has no inhibitory effect on tonic LH secretion PMID: 15127327
    7. findings provide strong evidence that the circannual regulation of prolactin secretion, and its associated biology, derive from a pituitary-based timing mechanism PMID: 17185605
    8. Prolactin expressed in adult and fetal sheep brain could be involved in neurodevelopment and/or modulation of the neuroendocrine stress axis. PMID: 18223310
    9. These results demonstrate that TGF-beta abrogates prolactin-stimulated beta-casein gene expression in mammary epithelial cells through, at least in part, a Smad3-dependent mechanism. PMID: 18335503
    10. circulating prolactin decreased (P<0.001) in response to leptin during long days. PMID: 18451065
    11. DNA polymorphism of 5' flanking region of prolactin gene and its association with litter size in sheep. PMID: 19207932
    12. PRL exerts protective effects on adult neurogenesis in the dentate gyrus under chronic stress conditions in mice. PMID: 19211889

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Somatotropin/prolactin family
  • 数据库链接：

    KEGG: oas:443317

    UniGene: Oar.390