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  4. Sheep 5-hydroxytryptamine/serotonin(5HT/ST) ELISA kit

Sheep 5-hydroxytryptamine/serotonin(5HT/ST) ELISA kit

  • 中文名称：
    綿羊5羟色胺/血清素/血清胺(5HT/ST)酶联免疫试剂盒
  • 货号：
    CSB-E14929Sh
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Sheep 5HT/ST ELISA Kit was designed for the quantitative measurement of Sheep 5HT/ST protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 0.625 ng/mL-10 ng/mL and the sensitivity is 0.312 ng/mL .

  • 缩写：
    5HT/ST
  • 种属：
    Ovis aries (Sheep)
  • 样本类型：
    serum, plasma
  • 检测范围：
    0.625 ng/mL-10 ng/mL
  • 灵敏度：
    0.312 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Biochemicals
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of sheep 5HT/ST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:20 Average % 84  
    Range % 80-92  
    1:40 Average % 97  
    Range % 91-105  
    1:80 Average % 96  
    Range % 90-101  
    1:160 Average % 93  
    Range % 86-98  
  • 回收率：
    The recovery of sheep 5HT/ST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-98  
    EDTA plasma (n=4) 97 90-100  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    10 0.122 0.115 0.119    
    5 0.234 0.216 0.225    
    2.5 0.404 0.407 0.406    
    1.25 0.743 0.739 0.741    
    0.625 1.221 1.194 1.208    
    0 2.051 1.959 2.005    
  • 数据处理：
  • 货期：
    3-5 working days

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