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  4. Rat visfatin ELISA Kit

Rat visfatin ELISA Kit

  • 中文名称：
    大鼠内脂素/内脏脂肪素(visfatin)酶联免疫试剂盒
  • 货号：
    CSB-E08941r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This rat visfatin ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of rat visfatin in different samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of visfatin bound in the initial step.

    Visfatin (NAMPT) is an adipocytokine that exerts many functions including enhancement of cell proliferation, biosynthesis of nicotinamide mono- and dinucleotide, and hypoglycaemic effect through an endocrine, autocrine, and paracrine manner. Visfatin binds to the insulin receptor at a site different from that of insulin, leading to hypoglycemia by reducing glucose release from liver cells and stimulating glucose utilization in adipocytes and myocytes. Visfatin is upregulated by hypoxia, inflammation, and hyperglycemia but downregulated by insulin, somatostatin, and statins. It is involved in many pathological processes, including obesity, diabetes, and cardiovascular diseases.

  • 别名：
    Nampt ELISA Kit; Pbef1 ELISA Kit; Nicotinamide phosphoribosyltransferase ELISA Kit; NAmPRTase ELISA Kit; Nampt ELISA Kit; EC 2.4.2.12 ELISA Kit; Pre-B-cell colony-enhancing factor 1 homolog ELISA Kit; PBEF ELISA Kit; Visfatin ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 ng/mL-200 ng/mL
  • 灵敏度：
    0.78 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat visfatin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %87
    Range %82-97
    1:2Average %96
    Range %92-103
    1:4Average %102
    Range %96-108
    1:8Average %94
    Range %89-98
  • 回收率：
    The recovery of rat visfatin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9590-100
    EDTA plasma (n=4)9894-108
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    2001.743 1.867 1.805 1.707
    1001.304 1.199 1.252 1.154
    500.835 0.838 0.837 0.739
    250.534 0.547 0.541 0.443
    12.50.348 0.336 0.342 0.244
    6.250.277 0.268 0.273 0.175
    3.120.171 0.177 0.174 0.076
    00.098 0.097 0.098 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression.
  • 基因功能参考文献：
    1. A Strong association was observed among fasting blood glucose, serum visfatin and trace elements levels of high fat diet and type 2 diabetes mellitus PMID: 29772226
    2. Our findings suggest that NAMPT inhibition may be a novel therapeutic approach for I/R-induced lung injury. The protective effects involve the suppression of multiple signal pathways. PMID: 28438162
    3. Thus, it can be suggested that visfatin may play an important role in testicular aging by regulating testicular steroidogenesis and spermatogenesis. PMID: 28163205
    4. visfatin plays a dual role in the apoptosis in rat Mesenteric lymph nodes, which is mediated by both the mitochondrial apoptotic pathway and the death-receptor apoptotic pathway. PMID: 26857566
    5. this study shows that serum visfatin levels, and white adipose tissue and hepatic visfatin mRNA levels of both male and female rats were increased by LPS injection PMID: 27083000
    6. Visfatin was expressed in the rats' retinas, mainly in the retinal nerve fibre layer, inner plexiform layer and outer plexiform layer. As the development of diabetes mellitus course, its expression was gradually increased. PMID: 26694625
    7. NAMPT protects against 6-hydroxydopamine-induced neurotoxicity in PC12 cells through modulating SIRT1 activity PMID: 27035562
    8. Report retinal toxicity of NAMPT inhibitors. PMID: 25505128
    9. visfatin could participate in the inflammatory process in rat spleen by modulating the macrophages and inflammatory cytokines. PMID: 25358398
    10. Visfatin induced the expression of alphaSMA in cultureactivated rat HSC, which was accompanied by a significant increase in collagen types I and III. PMID: 25351242
    11. These data are the first to show that Nampt inhibits myometrial contractility and does so more potently than leptin. PMID: 25645057
    12. Central visfatin improved glucose homeostasis by increasing insulin secretion and insulin sensitivity at euglycemia through the hypothalamus in diabetic rats. PMID: 24332931
    13. role in myocardial inflammation during endotoxemia PMID: 23831038
    14. Data indicate that nicotinamide phosphoribosyltransferase (NAMPT) mRNA and protein were increased in the kidneys with streptozotocin-induced diabetes. PMID: 24287278
    15. CR-induced beneficial effects in oxidative stress, mitochondrial biogenesis, and metabolic adaptation require NAMPT. PMID: 23946338
    16. visfatin plays a critical role in cardioprotection by suppressing myocardial apoptosis via AMPK activation PMID: 23065734
    17. Nampt promotes neuronal survival through inducing autophagy. PMID: 22113203
    18. Visfatin expression was increased mainly through the AT1-R-JAK/STAT pathway in the process of Ang II-induced cardiomyocyte hypertrophy. PMID: 21841463
    19. The expression of visfatin is closely associated with the expression of proinflammatory cytokines in FFA-induced inflammation and is significantly decreased by NF-kappaB inhibition in HepG2 cells. PMID: 21664630
    20. Our findings reveal that Nampt protects against ischemic stroke through rescuing neurons from death via the SIRT1-dependent AMPK pathway and indicate that Nampt is a new therapeutic target for stroke. PMID: 21246601
    21. results of the present study demonstrate that serum visfatin levels in Lyon hypertensive rats are elevated and are associated with lipid metabolic abnormalities PMID: 20456420
    22. No differences of serum visfatin levels were observed between spontaneously hypertensive rats and Wistar-Kyoto rats. Serum visfatin levels were significantly correlated to weights of skeletal muscle. PMID: 20658215
    23. Visfatin exhibits important autocrine effects on sensitivity of liver cells to insulin action possibly through its effects on NAD biosynthesis. PMID: 19929131
    24. Exercise training induced SIRT1 activity due to the positive effect of exercise on the activity of nicotinamide phosphoribosyltransferase (NAMPT) and thereby the production of sirtuin-fueling NAD(+). PMID: 19913571
    25. PBEF is not a cytokine-like secreted protein but an intracellular protein associated with the cell cycle PMID: 12782293
    26. relative visfatin gene expression is not associated with the metabolic syndrome in WOKW rats [Pbef, also called visfatin] PMID: 15922301
    27. Results describe the crystal structure of visfatin in three different forms: apo and in complex with either nicotinamide mononucleotide (NMN) or the nicotinamide phosphoribosyltransferase inhibitor FK-866. PMID: 16901503
    28. During fasting, increased Nampt provides protection against cell death and requires an intact mitochondrial NAD(+) salvage pathway as well as the mitochondrial NAD(+)-dependent deacetylases SIRT3 and SIRT4. PMID: 17889652
    29. Visfatin is produced in mesangial cells, which are a novel target for visfatin, and play an important role in the pathogenesis of diabetic nephropathy. PMID: 18768589
    30. improves insulin sensitivity and exerts its hypocholesterolemic effects at least partially through upregulation of the tyrosine phosphorylation of IRS-1 protein and the mRNA levels of PPARgamma and SREBP-2 PMID: 19263259
    31. The crystal structure of rat visfatin in complex with IS001, is desribed. PMID: 19533035
    32. Nicotinamide phosphoribosyltransferase regulates cell survival through NAD+ synthesis in cardiac myocytes. PMID: 19661458

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Secreted.
  • 蛋白家族：
    NAPRTase family
  • 组织特异性：
    Expressed in various tissues. At the highest level in liver and at the second highest in heart. The amount is higher in heart than in lung.
  • 数据库链接：