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  4. Rat vimentin (VIM)ELISA Kit

Rat vimentin (VIM)ELISA Kit

  • 中文名称：
    大鼠波形蛋白(VIM)酶联免疫试剂盒
  • 货号：
    CSB-E14029r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat VIM ELISA Kit was designed for the quantitative measurement of Rat VIM protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 ng/mL-1500 ng/mL and the sensitivity is 5.8 ng/mL.

  • 别名：
    Vim ELISA Kit; Vimentin ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    23.5 ng/mL-1500 ng/mL
  • 灵敏度：
    5.8 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat VIM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 88    
    Range % 80-95    
    1:2 Average % 93    
    Range % 88-98    
    1:4 Average % 97    
    Range % 93-102    
    1:8 Average % 100    
    Range % 95-104    
  • 回收率：
    The recovery of rat VIM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 102 95-107    
    EDTA plasma (n=4) 90 85-94    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    10 3.066 2.888 2.977 2.880    
    5 2.598 2.621 2.610 2.513    
    2.5 1.958 1.845 1.902 1.805    
    1.25 0.943 0.978 0.961 0.864    
    0.625 0.623 0.612 0.618 0.521    
    0.312 0.366 0.356 0.361 0.264    
    0.156 0.228 0.229 0.229 0.132    
    0 0.093 0.101 0.097 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Please provide the following information regarding Rat vimentin, VIM ELISA Kit (CSB-E14029r):
a. What is the Uniprot/Accession number that this kit is designed for? Is the kit designed for XYZ species or is it designed for a different species such as Human and cross reacts with XYZ species?
b. Is the capture antibody a monoclonal or polyclonal?
c. What is the host of the capture antibody?
d. Is the detection antibody a monoclonal or polyclonal?
e. What is the host of the detection antibody?
f. What’s is that standard? A recombinant protein or something else? If a recombinant protein, what is the host?
g. If recombinant protein, is it full length or partial?
h. What is the species of the standard– human or something else?
i. What sample type was tested with this kit (serum? plasma? Something else?) and what were the reference values.

A:
Thanks for your inquiry.
CSB-E14029r
This kit is designed against full length. Pls refer to: https://www.uniprot.org/uniprot/P31000.
Capture antibody is mouse monoclonal antibody.
Detection antibody is sheep polyclonal antibody.
Standard is NS0-derived recombinant protein.
This kit is designed for rat. Although it has homology with the protein from other species, we don't suggest you use this kit to test other species because there is matrix interference.
We tested serum and plasma samples before and the test value is 20--100ng/ml.
Pls let me know if you have any further questions. Thank you.

靶点详情

  • 功能：
    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.; Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • 基因功能参考文献：
    1. We labeled brain with ZO1, vimentin, pan-cadherin and angiotensin II type 1 receptors markers which showed a morphologically distinct population of cells at the region of the sulcus medianus organum similar to tanycytes present in the median eminence, a known circumventricular organ. PMID: 29803674
    2. In transected spinal cord rats, vimentin knockdown in the scar tissues showed a significant improvement on locomotor function in hindlimbs. PMID: 24874717
    3. The increased expression of LP-vimentin-IR cells suggests that changes in cell-cell interactions could play a role in ischemia-induced changes in bladder activity. PMID: 24728265
    4. Double-immunostaining experiments with antibodies against Stk33 and vimentin showed a striking colocalization of Stk33 and vimentin in the hypothalamus. PMID: 24057876
    5. We have identified three protein biomarkers (GSN, MYL2, and VIM) that have potential utility in diagnosing the severity of Chagas disease PMID: 22199233
    6. vimentin is a regulator of NRG1 type III function and peripheral nerve myelination PMID: 22357929
    7. Estradiol treatment leads to an effect on vimentin phosphorylation. PMID: 21915674
    8. These data suggest that the inability of YY1 to access the hypermethylated promoter may be one of the mechanisms that mediate vimentin downregulation. PMID: 22005459
    9. Data show that vimentin intermediate filaments play a crucial role in mechanical behavior not only at large deformations but also in the nanomechanical response of individual cells. PMID: 21426942
    10. Data show that astrocyte structural proteins GFAP and vimentin are induced and by chronic leptin administration, suggesting astrocytes participating in leptin modulated synaptic inputs. PMID: 21343257
    11. GR-mediated reduction in vimentin may be involved in the formation of steroid-induced cataract PMID: 21245955
    12. Increases in post-traumatic vimentin mRNA in the cortex and in the hippocampus appear together with vimentin immunoreactivity in astrocytes starting at one day after severe trauma. PMID: 20479526
    13. Endothelial cell hypoxia causes redistribution of vimentin to a more insoluble and extensive filamentous network that could play a role in endothelial barrier stabilization. PMID: 20427712
    14. Vimentin mRNA and protein expression levels increased significantly throughout hepatotumorigenesis. Vimentin is an important node in underlying gene regulatory networks. PMID: 20015863
    15. Fetal hepatocytes at the epithelial mesenchymal transition have a higher level of this protein than in fetal hepatocytes. PMID: 12496370
    16. induction in glial cells of hippocampus following hyperthermia PMID: 12834255
    17. vimentin may play a key role in the formation of glial scars in the central nervous system PMID: 15684657
    18. demonstrate the alterations of alpha-smooth muscle actin and vimentin localization patterns in the bovine cryptorchid testis as compared to the contralateral scrotal testis during postnatal development PMID: 16493181
    19. Taken together, these results suggest that vimentin is a novel member of binding partner of PP1c in astrocytes, and vimentin-Ser82 may act as a memory phosphorylation site. PMID: 16629905
    20. Vimentin phosphorylation was increased, indicating an accumulation of phosphorylated vimentin associated to the cytoskeleton in hyperthyroidism induced rats. PMID: 17306450
    21. Rats fed the homocysteine diet had an increase in Vim. PMID: 17485853
    22. vimentin-immunopositive cells localize (in both intact and lesioned brain) in the zone of barrier structures' formation. PMID: 18198667
    23. the phosphorylation and the immunoreactivity of cytoskeletal-associated vimentin were increased without altering vimentin expression, suggesting an accumulation of insoluble and phosphorylated vimentin. PMID: 18316471
    24. Single impact load also causes changes in the pattern of vimentin immunoreactivity, indicating vimentin dissassembley in cartilage injury model PMID: 18577232
    25. Vimentin was expressed in cells of the outer and inner capsules of all muscle spindles and in newly formed myoblasts and myotubes of regenerating spindles 7 days after grafting PMID: 18941770
    26. Vimentin was observed within cells located in the primitive ducts of rat pancreas newborn animals. It was also spotted in alpha cells starting from E12.5 but disappeared soon after birth, likely identifying immature or recently differentiated alpha cells. PMID: 19223297

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  • 亚细胞定位：
    Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix. Cell membrane.
  • 蛋白家族：
    Intermediate filament family
  • 数据库链接：

    KEGG: rno:81818

    STRING: 10116.ENSRNOP00000024430

    UniGene: Rn.2710