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  4. Rat urokinase plasminogen activator,uPA ELISA kit

Rat urokinase plasminogen activator,uPA ELISA kit

  • 中文名称：
    大鼠尿激酶型纤溶酶原激活物(uPA)酶联免疫试剂盒
  • 货号：
    CSB-E07368r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat PLAU ELISA Kit was designed for the quantitative measurement of Rat PLAU protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名：
    PlauUrokinase-type plasminogen activator ELISA kit; U-plasminogen activator ELISA kit; uPA ELISA kit; EC 3.4.21.73) [Cleaved into: Urokinase-type plasminogen activator long chain A; Urokinase-type plasminogen activator short chain A; Urokinase-type plasminogen activator chain B] ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat uPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 91    
    Range % 88-95    
    1:2 Average % 95    
    Range % 90-100    
    1:4 Average % 97    
    Range % 94-103    
    1:8 Average % 101    
    Range % 96-105    
  • 回收率：
    The recovery of rat uPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 90 87-94    
    EDTA plasma (n=4) 96 94-101    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    1000 2.334 2.186 2.260 2.077    
    500 1.652 1.625 1.639 1.456    
    250 1.047 0.997 1.022 0.839    
    125 0.636 0.688 0.662 0.479    
    62.5 0.398 0.402 0.400 0.217    
    31.2 0.325 0.337 0.331 0.148    
    15.6 0.246 0.232 0.239 0.056    
    0 0.189 0.177 0.183 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family
  • 数据库链接：