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  4. Rat tissue inhibitors of metalloproteinase 2,TIMP-2 ELISA Kit

Rat tissue inhibitors of metalloproteinase 2,TIMP-2 ELISA Kit

  • 中文名称：
    大鼠基质金属蛋白酶抑制因子2(TIMP-2)酶联免疫试剂盒
  • 货号：
    CSB-E07386r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    The Rat tissue inhibitors of metalloproteinase 2 (TIMP-2) ELISA Kit is an essential tool for cancer researchers.Protein TIMP-2 can be used as a reference index in the diagnosis of organ injury, early tumor and prognosis. The kit is specifically designed to detect Metalloproteinase inhibitor 2 protein in serum, plasma, and tissue homogenates of Rattus norvegicus (Rat) species.

    The TIMP-2 ELISA Kit has a wide detection range of 1.25 ng/mL - 80 ng/mL and an impressive sensitivity of 0.31 ng/mL. This ensures accurate and precise quantification of TIMP-2 protein levels in your samples. The assay time is quick, ranging from 1-5 hours, and requires a small sample volume of 50-100ul.

    The kit utilizes a sandwich assay principle, which guarantees high specificity and sensitivity. The detection wavelength is set at 450 nm, allowing optimal accuracy and reproducibility of results. The TIMP-2 ELISA kit has been cited in over four publications, attesting to its reliability and accuracy.

    The TIMP-2 ELISA Kit is an essential tool for researchers in the cancer research area. The quantitative measurement of TIMP-2 levels in rat samples allows researchers to study the role of Metalloproteinase inhibitor 2 in cancer development and progression. The kit offers accurate and reliable results, making it an essential tool in cancer research.

  • 别名：
    Timp2 ELISA Kit; Timp-2 ELISA Kit; Metalloproteinase inhibitor 2 ELISA Kit; Tissue inhibitor of metalloproteinases 2 ELISA Kit; TIMP-2 ELISA Kit
  • 缩写：
    TIMP2
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.25 ng/mL-80 ng/mL
  • 灵敏度：
    0.31 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TIMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 91
    Range % 88-95
    1:2 Average % 95
    Range % 90-100
    1:4 Average % 97
    Range % 94-103
    1:8 Average % 101
    Range % 96-105
  • 回收率：
    The recovery of rat TIMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 90 87-94
    EDTA plasma (n=4) 96 94-101
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    80 2.184 2.189 2.187 2.009
    40 1.486 1.456 1.471 1.293
    20 0.925 0.994 0.960 0.782
    10 0.545 0.588 0.567 0.389
    5 0.431 0.443 0.437 0.259
    2.5 0.308 0.292 0.300 0.122
    1.25 0.251 0.233 0.242 0.064
    0 0.179 0.177 0.178 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Can you please provide the following information about this kit:
a. Capture antibody or capture antigen.
b. Detection antibody (if applicable).
c. Standard – if recombinant protein, please specify sequence (including species source and accession no. if available) and expression host.
4) If you have data on the normal value and the value in case of renal failure of TIMP-2 in rats with the kit, please send me too.
5) Please send the current manual.

A:
Thanks for your inquiry.
CSB-E07386r
This kit is designed against full length protein. Pls refer to: https://www.uniprot.org/uniprot/P30121.
Capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody.
Standard is NS0-derived recombinant protein.
We tested undiluted serum and plasma samples before and the test value is 2.5--10ng/ml.
I will send you the manual by email.

靶点详情

  • 功能：
    Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.
  • 基因功能参考文献：
    1. Dynamics of distribution of capillaries with MMP2 and TIMP2 expressed in rat brain during development of experimental hypertension has been reported. PMID: 29308554
    2. During the processes of conjunctival filtering bleb and scar formation in rats, the changes in MMP-2 and TIMP-2 levels in the filtering area, together with a corresponding proliferation of fibroblasts and the accumulation of collagen fibres, resulted in scarring of filtering blebs. PMID: 29075637
    3. These findings suggested that artesunate attenuates alveolitis and pulmonary fibrosis by regulating expression of collagen-IV, TIMP-1 and 2, as well as MMP-2 and -9, to reduce ECM accumulation. PMID: 27323108
    4. Data suggest that amlodipine and Atorvastatin could improve ventricular remodeling in SHR rats through intervention with the imbalance of MMP-2/TIMP-2 and MMP-9/TIMP-1 system. PMID: 27322513
    5. decreased in diabetic skin PMID: 26047379
    6. Lung tissue from burn animals showed increased matrix metalloproteinase activity, decreased levels of TIMP-2, and no difference in levels of active MMP-9 in burn vs. control groups. PMID: 26601648
    7. MMP-2 and MMP-9 activities and TIMP-1 and TIMP-2 expression were significantly reduced in the lateral prostatic lobe of the ethanol drinking animals. PMID: 26258010
    8. The present study suggests that EPO can exert a cardioprotective effect on DOX-induced cardiotoxicity which may be associated with improving MMP-2/TIMP-2 imbalance. PMID: 24685074
    9. Enhancement of TIMP-2 expression may be a potential therapeutic target for neuroinflammatory disorders. PMID: 24970341
    10. Bone marrow mononuclear cell transplantation significantly increased MMP-9 and MMP-13, and decreased TIMP expression. PMID: 23886643
    11. the expression of TIMP-2 increased only in the neutrophils, endotheliocytes, and fibroblasts, remaining to be equal over all stages. PMID: 23383440
    12. Membrane type 1 MMP, MMP2, and TIMP2 were present in testis during gonocyte migration and transformation into spermatogenic stem cells. PMID: 22974613
    13. Inulicin inhibits hyperplasia of neointima by modulating the balance of MMP-2 and TIMP-2. PMID: 21141515
    14. Data show that MMPs 2 and 9 and their inhibitors, TIMPs 1 and 2, are upregulated in response to oxidative stress produced by HIV-1 gp120 in a rat model of HIV encephalopathy. PMID: 22092673
    15. NDRG2 contributed to an increase in the ratio of matrix metalloproteinase 2 (MMP2) to tissue inhibitor of matrix metalloproteinase 2 PMID: 22110735
    16. Trained spontaneously hypertensive rats showed higher metalloproteinase-2/tissue inhibitor metalloproteinase-2 (MMP-2/TIMP-2) ratio and lower levels of alpha-smooth muscle actin. PMID: 22045123
    17. A dramatic increase of TIMP1 mRNA and a decrease of TIMP2 in DRG after sciatic nerve transection. PMID: 21782897
    18. the present study showed for the first time the mode of TIMP-1 and -2 expression and activity in a rat liver fibrosis model. PMID: 20226641
    19. After transfection with FRNK, MMP-2 was up-regulated and TIMP-2 was down-regulated in hepatic stellate cells. PMID: 18983773
    20. The TIMP-2 gene mediated by adenovirus can renew the balance of degradation of the extracellular matrix caused by elastase and inhibit the formation of an abdominal aortic aneurysm. PMID: 20103929
    21. Addition of follicle stimulating hormone to Sertoli cells increased the TIMP-2 mRNA level three-fold, implicating a role in spermatogenesis. PMID: 11928819
    22. Data show that matrix metalloproteinase-2 and TIMP-2 are likely involved in the FSH-induced changes in Sertoli cells. PMID: 12039062
    23. tissue inhibitor of metalloproteinase-2 mRNA was observed surrounding the developing corpus luteum and was present in granulosa-lutein cells, with high levels near the center of the corpus luteum PMID: 12444073
    24. results suggest that the migration and invasion of cardiac fibroblasts is furin-dependent and that the active species of MT1-MMP and MMP-2 may be involved in both events PMID: 12970340
    25. In the process of hepatic fibrosis, fibroblasts and myofibroblasts are the major cells that express TIMPs and the more serious the hepatic fibrosis is in the injured liver, the higher the level of TIMP-1 and TIMP-2 gene expression. PMID: 14695775
    26. Blood leukocytes and loss of flow are major factors in the activation of MMP-2, and cytokine-mediated differential regulation of TIMP-1 and TIMP-2 may contribute significantly to blood brain barrier failure. PMID: 16707552
    27. MMP2 plays a role in the development of BC metastases. Furthermore, our results suggest that astrocyte factors and the ERK1/2 signaling pathway may be associated with BC brain metastasis development. PMID: 17505812
    28. matrix metalloproteinase-2, tissue inhibitor of metalloproteinase-1 and osteopontin have roles in heart failure in DOCA-salt hypertensive rat PMID: 18415800
    29. TIMP-2 is an osteoanabolic factor in vitro and in vivo PMID: 18558556
    30. The induction of MMP-2, MMP-9, TIMP-1 and TIMP-2 mRNAs post-castration could be at least in part due to post-transcriptional stabilization. PMID: 18675881
    31. investigated the involvement of matrix metalloproteinases (MMPs), tissue inhibitor (TIMP) and endothelin-1 (ET-1) in the renal damage in spontaneously hypertensive rats following nitric oxide deprivation. PMID: 18935914
    32. over-expressed ZAK may contribute to the progression of myocardial remodeling by disrupting the balance in the MMP-2/9 and TIMP-1/2 expression level, respectively PMID: 19184368

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Protease inhibitor I35 (TIMP) family
  • 数据库链接：