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Rat tissue factor(TF)/coagulation factor VII(F7) complex ELISA kit

  • 中文名称:
    大鼠组织因子与活化凝血因子7复合物酶联免疫试剂盒
  • 货号:
    CSB-EQ027252RA
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:

    The Rat Tissue Factor (TF)/Coagulation Factor VII (F7) Complex ELISA Kit is the perfect solution for researchers studying the cardiovascular system. This quantitative assay kit allows for the detection of TF/F7 complex in serum, plasma, and tissue homogenates of Rattus norvegicus (rat) samples.

    With a detection range of 15.6 pg/mL to 1000 pg/mL and a sensitivity of 3.9 pg/mL, this ELISA kit provides accurate and reliable results for your research needs. The assay time is quick, ranging from 1-5 hours, and the sample volume required is only 50-100 ul.

    The detection wavelength for this kit is 450 nm, providing high sensitivity and specificity for the measurement of TF/F7 complex in rat samples. The assay principle is based on the sandwich method, providing high specificity and accuracy for the detection of this complex.

    The Rat Tissue Factor (TF)/Coagulation Factor VII (F7) Complex ELISA Kit is ideal for researchers working in the cardiovascular field, and provides a powerful tool for the study of TF/F7 complex in rat samples.

  • 缩写:
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    15.6 pg/mL-1000 pg/mL
  • 灵敏度:
    3.9 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cardiovascular
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TF/F7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 107
    Range % 98-110
    1:2 Average % 103
    Range % 96-107
    1:4 Average % 98
    Range % 89-102
    1:8 Average % 105
    Range % 98-110
  • 回收率:
    The recovery of rat TF/F7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 91 85-96
    EDTA plasma (n=4) 102 95-107
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1000 2.708 2.673 2.691 2.589
    500 2.243 2.150 2.197 2.095
    250 1.419 1.469 1.444 1.342
    125 0.702 0.734 0.718 0.616
    62.5 0.412 0.425 0.419 0.317
    31.2 0.251 0.263 0.257 0.155
    15.6 0.167 0.172 0.170 0.068
    0 0.101 0.103 0.102  
  • 数据处理:
  • 货期:
    3-5 working days

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