Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Rat tight junction protein 1 (ZO1) ELISA Kit

Rat tight junction protein 1 (ZO1) ELISA Kit

  • 中文名称：
    大鼠紧密连接蛋白1(ZO1)酶联免疫试剂盒
  • 货号：
    CSB-E17287r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat ZO1 ELISA Kit was designed for the quantitative measurement of Rat ZO1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

  • 别名：
    DKFZp686M05161, MGC133289, ZO-1, tight junction protein 1|tight junction protein ZO-1|zona occludens 1|zonula occludens 1 protein
  • 缩写：
    ZO1
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Tags & Cell Markers
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat ZO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 86-95  
    1:2 Average % 102  
    Range % 97-107  
    1:4 Average % 91  
    Range % 85-97  
    1:8 Average % 97  
    Range % 91-103  
  • 回收率：
    The recovery of rat ZO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-98  
    EDTA plasma (n=4) 97 90-100  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2000 2.272 2.374 2.323 2.221  
    1000 1.846 1.967 1.907 1.805  
    500 1.496 1.392 1.444 1.342  
    250 0.962 0.946 0.954 0.852  
    125 0.578 0.596 0.587 0.485  
    62.5 0.364 0.351 0.358 0.256  
    31.2 0.225 0.230 0.228 0.126  
    0 0.101 0.102 0.102    
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价