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  4. Rat thyrotropin-releasing hormone,TRH ELISA KIT

Rat thyrotropin-releasing hormone,TRH ELISA KIT

  • 中文名称：
    大鼠促甲状腺素释放激素(TRH)酶联免疫试剂盒
  • 货号：
    CSB-E08040r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    The Rat Thyrotropin-Releasing Hormone (TRH) ELISA Kit is a highly sensitive and accurate assay for the detection and quantification of TRH in serum, plasma, and tissue homogenates from the Rat species.

    TRH is a peptide hormone that plays a vital role in regulating the release of thyroid-stimulating hormone (TSH) from the pituitary gland. Abnormalities in TRH levels have been linked to a variety of neurological and psychiatric disorders, as well as endocrine system dysfunction. Thyroid hormone-releasing hormone is the first messenger signal in the brain in many of the actions that control thyroid hormone production.Our TRH ELISA Kit offers a simple and efficient means of measuring TRH levels in biological samples, with a detection range of 0.5 pg/mL to 100 pg/mL and a sensitivity of 0.25 pg/mL.

    The assay principle of our TRH ELISA Kit is based on the competitive method. During the assay process, a TRH-specific antibody is added to the coated wells, along with a biotinylated TRH standard and the sample. The biotinylated TRH competes with the TRH in the sample for binding to the antibody, resulting in a decreased signal. The signal is then detected using streptavidin-HRP and a chromogenic substrate, producing a colorimetric signal that is inversely proportional to the TRH concentration.

    This TRH ELISA Kit offers a quick assay time of 1-5 hours, with a sample volume of 50-100ul and a detection wavelength of 450 nm. It is suitable for use in signal transduction and endocrine system research and has been cited in more than 2 publications.

  • 别名：
    Trh ELISA Kit; Pro-thyrotropin-releasing hormone ELISA Kit; Pro-TRH ELISA Kit; Prothyroliberin) [Cleaved into: Thyrotropin-releasing hormone ELISA Kit; TRH ELISA Kit; Protirelin ELISA Kit; TSH-releasing factor ELISA Kit; Thyroliberin ELISA Kit; Thyrotropin-releasing factor ELISA Kit; TRF)] ELISA Kit
  • 缩写：
    TRH
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.5 pg/mL-100 pg/mL
  • 灵敏度：
    0.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 100  
    Range % 96-104  
    1:2 Average % 92  
    Range % 88-95  
    1:4 Average % 93  
    Range % 87-96  
    1:8 Average % 101  
    Range % 97-105  
  • 回收率：
    The recovery of rat TRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-99  
    EDTA plasma (n=4) 97 93-102  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    100 0.150 0.146 0.148    
    25 0.301 0.298 0.300    
    8 0.487 0.500 0.494    
    2 0.776 0.790 0.783    
    0.5 1.264 1.288 1.276    
    0 1.932 1.907 1.920    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems.
  • 基因功能参考文献：
    1. Intrauterine Zn deficiency favors thyrotropin-releasing hormone-increasing effects on thyrotropin serum levels and induces subclinical hypothyroidism in weaned rats. PMID: 29057835
    2. We found that dehydration induced anorexia rats showed increased number of thyrotropin-releasing hormone (TRH) cells in caudal paraventricular hypothalamic nucleus, as well as a decreased percentage of TRH-expressing neurons that co-expressed monocarboxylate transporter-8 mRNA signal. PMID: 26626087
    3. The results of this study concluded that long-term changes in dietary Zn down-regulate PPII activity PMID: 26315400
    4. Delayed increases in both the median eminence PPII as well as the thyroliberinase activities in fasted male rats may facilitate the maintenance of deep downregulation of the HPT axis function, despite a partial reactivation of TRH expression in the PVN. PMID: 25942072
    5. TGFbeta2 regulates hypothalamic TRH expression through TIEG1 during fetal development. PMID: 25448845
    6. a specific TRH increase in the left ventricle induced structural changes in the normal heart PMID: 25281568
    7. Trh, Tnxa and Nnat genes were affected according to the degree of memory in male rats. PMID: 24370184
    8. TRH upregulates the phenotypes of dermal fibroblasts and plays a role in accelerating wound healing. PMID: 24713470
    9. Early-overfed rats presented low proTRH and proCRT synthesis in paraventricular nucleus. PMID: 24464021
    10. Dysfunctional brainstem thyrotropin-releasing hormone (TRH)-elicited vagal impairment contributes to disturbed food intake in type 2 diabetic Goto-Kakizaki rats. PMID: 23701881
    11. NPY (neuropeptide Y) regulates TRH (1) by opposing alpha-MSH (alpha-melanocyte-stimulating hormone) activation of CREB signaling and reducing pro-TRH processing by proprotein convertase 2 and (2) by reducing production/release of alpha-MSH. PMID: 23321476
    12. TRH neurons heavily innervate histaminergic neurons in all subdivisions of the tuberomammillary nucleus. PMID: 23063458
    13. Food-restricted and dehydrated-induced anorexic rats present differential TRH expression in anterior and caudal PVN. Role of type 2 deiodinase and pyroglutamyl aminopeptidase II. PMID: 22719053
    14. E(2) may act as a modulator of the secretory response of lactotrophs induced by TRH through membrane estrogen receptors, with the contribution by PI3K/Akt pathway activation. PMID: 22354782
    15. genes that might participate in the development and function of hypothalamic TRH neurons PMID: 21569245
    16. Klf4 plays a key role in the maturation of TRH expression in hypothalamic neurons. PMID: 21182892
    17. Data suggest that 17b-oestradiol negatively regulates the in vivo expression of thyrotrophin-releasing hormone mRNA in the paraventricular nucleus. PMID: 19302192
    18. These results corroborate the CRE-2 site as the main cAMP-response element of rat TRH promoter. PMID: 21266205
    19. Data suggest a TRH-mediated regulatory mechanism that may underlie the pathologic neuroplasticity driving dopamine hyper-responsivity in Parkinson's disease. PMID: 21085660
    20. These results show for the first time that the cardiac TRH system is involved in the development of left ventricular hypertrophy in SHR. PMID: 21135357
    21. Interaction of cAMP- and glucocorticoid-mediated regulation of TRH transcription at the CRE and cGRE regions of the TRH promoter. PMID: 20136691
    22. results show that pCREB binds to a response element in the TRH promoter (CRE-2) that is independent of Site-4 where thyroid hormone receptor (TR) beta2 is bound; pCREB and TR do not present mutual interference on their binding sites PMID: 19602869
    23. TRH-leptin interaction may contribute to the strong association between hypertension and obesity. PMID: 11882596
    24. Adenohypophyseal pyroglutamyl aminopeptidase II activity and mRNA levels fluctuate during the day and TRH down-regulates this activity in vivo PMID: 12213674
    25. CART expressed in hypophysiotropic TRH neurons may have important role in modulation of TRH-induced prolactin secretion. Increased secretion of CART may cause reduced TRH-induced prolactin response during hypothyroidism. PMID: 14691017
    26. results show that that leptin can regulate trh gene expression via activation of intracellular signal transducer and activator of transcription 3 (STAT3) proteins in TRH neurons in the hypothalamus PMID: 14764629
    27. TRH is specifically induced in the heart after MI and increases cardiac performance in rats with ischemic cardiomyopathy. Pro-TRH/TRH may be another important axis that affects hemodynamics and cardiac function in heart failure. PMID: 15096458
    28. central role of the IB1/JIP-1 protein in the control of TRH-mediated TSH-beta stimulation. PMID: 15345675
    29. Occurrence of VGLUT2 immunoreactivity within TRH and CRH axon varicosities, suggesting terminal glutamate release from these neuroendocrine systems. Hypophysiotropic TRH and CRH neurons possess glutamatergic characteristics. PMID: 15486233
    30. suppression of thyrotropin-releasing hormone gene expression in the hypothalamic paraventricular nucleus by lipopolysaccharide is not caused by brainstem pathways PMID: 15604205
    31. transcription regulated by rapid cross-talk of glucocorticoids with PKA signaling pathways PMID: 15691887
    32. TRH plays an important role in the mechanism of insulin secretion and its response to glucose stimulation. PMID: 15750838
    33. three neuronal phenotypes in the hypothalamic structure that is involved in the induction of attacks: glutamatergic neurons co-expressing thyrotropin releasing hormone, glutamatergic neurons without thyrotropin releasing hormone, and GABAergic neurons PMID: 15908131
    34. Data show that suppression of deiodinase type II (DII) by iopanoic acid during fasting prevented elevated DII activity and blunted the decline in hypothalamic thyrotropin releasing hormone mRNA levels. PMID: 16098513
    35. TRH-like peptides are rapidly modulated in rat brain by thyroid hormones PMID: 16310891
    36. in the hypothalamic paraventricular nucleus there are subpopulations of proTRH neurons responding to leptin, which is dependent upon the way leptin reaches its primary target(s) in the hypothalamus PMID: 16627588
    37. Physiological role of brainstem TRH in vagal-ghrelin-mediated stimulation of food intake, which involves interaction with brainstem Y1 receptors. PMID: 16959836
    38. K8 is a physiological substrate for PKCepsilon, and the phosphorylation at Ser8 and Ser23 transduces, at least in part, TRH-PKCepsilon signaling in pituitary cells PMID: 17553064
    39. Novel information on the molecular mechanisms of control of hypophysiotropic TRH biosynthesis. PMID: 17584968
    40. Results describe the modulation of thyrotropin-releasing hormone (TRH) and TRH-like peptide levels in rat brain and endocrine organs by lipopolysaccharide. PMID: 17726229
    41. These results indicate that hypothalamic neuronal histamine mediates the TRH-induced suppression of feeding behavior. PMID: 17760865
    42. BDNF may contribute to the enhancement of pre-pro-TRH mRNA expression in the hypothalamic paraventricular hypothalamic nucleus during development PMID: 17854778
    43. Response to cold stimulation leading to increased pro-TRH mRNA levels and TRH release was preserved under reduced energy availability in food restrictioned but dehydration-anorexic rats. PMID: 18191132
    44. Data show that protein kinase C and ERK signaling modulate glucocorticoid receptor activity and its interaction with CREB or AP-1 at the TRH gene promoter. PMID: 18427988
    45. Fasting reduced TRH mRNA compared with fed animals in hypothalamic paraventricular nucleus. PMID: 18467436
    46. pro-TRH-derived peptides are differentially sorted within the secretory pathway and that the initial cleavage in the trans-Golgi network is key to this process PMID: 18474603
    47. N-terminal domain within the prohormone sequence does not act as "sorting signal" in late secretion; instead, it seems to play a key role determining the proper folding pathway of the precursor and, thus, its stability PMID: 18779326
    48. TRH acts as a stimulator of vasopressin biosynthesis most of all in young male rats and as an inhibitor for oxytocin biosynthesis especially in mature animals. PMID: 19617647
    49. Released TRH is inactivated by an ectopeptidase, pyroglutamyl aminopeptidase II (PPII). Acute administration of alcohol modulates pyroglutamyl amino peptidase II activity and mRNA levels in rat limbic regions. PMID: 15707699

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    TRH family
  • 数据库链接：

    KEGG: rno:25569

    STRING: 10116.ENSRNOP00000015944

    UniGene: Rn.22