Rat platelet-derived growth factor-BB,PDGF-BB ELISA Kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

This Rat PDGF-BB ELISA Kit was designed for the quantitative measurement of Rat PDGF-BB protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 39.063 pg/mL-2500 pg/mL and the sensitivity is 9.77 pg/mL.
缩写：

PDGF-BB
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

39.063 pg/mL-2500 pg/mL
灵敏度：

9.77 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PDGF-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	104
	Range %	101-107
1:2	Average %	97
	Range %	94-100
1:4	Average %	88
	Range %	83-90
1:8	Average %	89
	Range %	84-93

回收率：

The recovery of rat PDGF-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	105	101-109
EDTA plasma (n=4)	110	107-113

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.