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  4. Rat placenta growth factor,PLGF ELISA kit

Rat placenta growth factor,PLGF ELISA kit

  • 中文名称：
    大鼠胎盘生长因子(PLGF)酶联免疫试剂盒
  • 货号：
    CSB-E07400r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The Rat placenta growth factor(PLGF)ELISA Kit allows for the in vitro quantitative determination of PLGF concentrations in serum, plasma, or tissue homogenates. The detection mechanism of this kit is based on the Sandwich-ELISA technique. PLGFin the sample is bound to the anti-rat PLGFantibody immobilized on the microtiter plate and then sandwiched with a Biotin-labeled PLGFantibody. The solution color develops into blue after the ordinal addition of HRP-avidin and TMB. The color development is terminated after adding the stop solution, and the color turns from blue to yellow. The color intensity is positively correlated with PLGFcontent in the sample. This kit exclusively recognizes rat PLGF. The kit has been quality controlled with high sensitivity, strong specificity, good linearity, precision of less than 10%, recovery of 89%-98%, and high lot-to-lot consistency. Refer to the product manual for more information.

    PLGF is a member of the VEGF family and is mainly expressed in the placenta. It is a crucial molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. Specifically, PLGF plays a role in trophoblast growth and differentiation. In human atherosclerotic lesions, PLGF expression is linked to plaque inflammation and neovascular growth. PLGF is also a potential biomarker for pre-eclampsia, a pregnancy complication characterized by hypertension and proteinuria in pregnancy.

  • 别名：
    Pgf ELISA Kit; Plgf ELISA Kit; Placenta growth factor ELISA Kit; PlGF ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 pg/mL-200 pg/mL
  • 灵敏度：
    0.78 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PLGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 88-95  
    1:2 Average % 94  
    Range % 89-98  
    1:4 Average % 93  
    Range % 87-97  
    1:8 Average % 99  
    Range % 93-102  
  • 回收率：
    The recovery of rat PLGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 89-97  
    EDTA plasma (n=4) 94 91-98  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    200 2.246 2.234 2.240 2.085  
    100 1.472 1.456 1.464 1.309  
    50 0.901 0.914 0.908 0.753  
    25 0.639 0.649 0.644 0.489  
    12.5 0.364 0.389 0.377 0.222  
    6.25 0.300 0.308 0.304 0.149  
    3.12 0.252 0.242 0.247 0.092  
    0 0.156 0.154 0.155 　  
  • 本试剂盒所含材料：
    • A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat PLGF antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled PLGF antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
    • One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
    • One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
    • An instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Also promotes cell tumor growth.
  • 基因功能参考文献：
    1. Studied the influence of dendrobium nobile Lindley (DNL) extract and its bibenzyl component moscatilin on the expression of placental growth factor (PLGF) and Norrie disease protein (NDP) in hypoxia model in retinal cells. Found DNL and moscatilin are able to protect against retinal ischemic/hypoxic changes respectively by downregulating PLGF and upregulating NDP. PMID: 29933759
    2. this paper show that knockdown of PLGF mitigates damage in hyperoxic lung by blocking of NFkappaB signaling PMID: 27280587
    3. PLGF triggers focal adhesion disassembly in alveolar type II cells via inhibiting the activation of focal adhesion kinase. PMID: 25515701
    4. PlGF had a positive feedback regulation with 15-lipoxygenase expression and 15-hydroxyeicosatetraenoic acid generation. PMID: 24001991
    5. placental gene expression is modulated by gestational conditions that might disrupt the fetal growth PMID: 23691181
    6. PlGF repairs myocardial ischemia through mechanisms of angiogenesis, cardioprotection and recruitment of myo-angiogenic competent marrow progenitors PMID: 21969865
    7. augmentation of brain Placental growth factor is associated with development of epilepsy. PMID: 22079325
    8. PLGF expression in the lung tissue in rats with paraquat-induced pulmonary fibrosis is upregulated. PMID: 17997886
    9. This is the first demonstration that sustained intraocular PGF production induces vascular and retinal changes similar to those observed in the early stages of diabetic retinopathy. PMID: 21408222
    10. Intramyocardial injections of autologous myoblasts overexpressing placental growth factor improved cardiac function, attenuated adverse cardiac remodeling, induced angiogenesis, and probably enhanced survival of grafted myoblasts. PMID: 21329947
    11. This study shows for the first time the placental growth factor upregulation in regenerating livers, which is related to hypoxia stimulation and liver growth. PMID: 21088600
    12. Endothelial cell-released H2O2 acts as a positive regulator of placenta growth factor gene and protein expression in vascular smooth muscle cells. PMID: 21123739
    13. The ARPE19 human junctions in culture are modulated by VEGF through VEGFR-1 but not through VEGFR-2. PMID: 18421240
    14. The levels of PlGF-2 in non-malformed embryos of diabetic gestation and their placentas were significantly (P < 0.05) lower than the average of controls. PMID: 19048427

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    PDGF/VEGF growth factor family
  • 数据库链接：

    KEGG: rno:94203

    STRING: 10116.ENSRNOP00000007790

    UniGene: Rn.6960