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  4. Rat pigment epithelium-derived factor,PEDF ELISA Kit

Rat pigment epithelium-derived factor,PEDF ELISA Kit

  • 中文名称：
    大鼠色素上皮衍生因子(PEDF)酶联免疫试剂盒
  • 货号：
    CSB-E08819r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat PEDF ELISA Kit was designed for the quantitative measurement of Rat PEDF protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

  • 缩写：
    PEDF
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PEDF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1000Average %87
    Range %81-92
    1:2000Average %95
    Range %91-99
    1:4000Average %92
    Range %85-97
    1:8000Average %96
    Range %91-102
  • 回收率：
    The recovery of rat PEDFspiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9289-95
    EDTA plasma (n=4)8783-91
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.758 2.615 2.687 2.549
    2002.065 1.968 2.017 1.879
    1001.357 1.397 1.377 1.239
    500.846 0.831 0.839 0.701
    250.572 0.581 0.577 0.439
    12.50.354 0.334 0.344 0.206
    6.250.264 0.256 0.260 0.122
    00.139 0.137 0.138
  • 数据处理：
  • 货期：
    3-5 working days

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■ 常见问题解答
Q:

Please provide the following information regarding Rat pigment epithelium-derived factor, PEDF ELISA Kit (CSB-E08819r):
a. Can cell culture lysates/supernatants samples be used with this kit? Have cell culture lysates/supernatants samples been tested with this kit?
b. If yes, what were the observed concentration values?
c. If they have not, would this assay be compatible with cell culture lysates/supernatants samples? Is there a recommended protocol to use with cell culture lysates/supernatants samples?

A:
Thanks for your inquiry.
The kit can be used for cell culture supernatants matrix.
However, we have no detection on the sample. If the sample content is under the detection range, it can be detected well.