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中文名称:大鼠神经肽Y(NPY)酶联免疫试剂盒
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货号:CSB-E13431r
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规格:96T/48T
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价格:¥3900/¥2500
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其他:
产品详情
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产品描述:
This Rat NPY ELISA Kit was designed for the quantitative measurement of Rat NPY protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL .
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缩写:
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Uniprot No.:
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种属:Rattus norvegicus (Rat)
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样本类型:serum, plasma, tissue homogenates
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检测范围:1.56 pg/mL-100 pg/mL
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灵敏度:0.39 pg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Neuroscience
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat NPY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 92-104 1:2 Average % 97 Range % 92-103 1:4 Average % 93 Range % 89-95 1:8 Average % 86 Range % 82-92 -
回收率:
The recovery of rat NPY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 93 87-99 EDTA plasma (n=4) 105 100-110 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 100 2.487 2.385 2.436 2.290 50 1.629 1.527 1.578 1.432 25 0.916 0.937 0.927 0.781 12.5 0.574 0.565 0.570 0.424 6.25 0.375 0.386 0.381 0.235 3.12 0.261 0.250 0.256 0.110 1.56 0.186 0.182 0.184 0.038 0 0.146 0.145 0.146 -
数据处理:
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货期:3-5 working days
引用文献
- Djulis Hull Enhances the Efficacy of Ferric Citrate Supplementation via Restoring Normal Iron Efflux through the IL-6–Hepcidin–Ferroportin Pathway in High-Fat-Diet … A Faradina,Journal of agricultural and food chemistry,2023
- Saponin extract of Baihe-Zhimu Tang ameliorates depression in chronic mild stress rats D Jia,Journal of Functional Foods,2020
- Increased Oxidative Damage Contributes to Mitochondrial Dysfunction in Muscle of Depressed Rats Induced by Chronic Mild Stress Probably Mediated by SIRT3 … Q Xiao,ANIMAL AND HUMAN PHYSIOLOGY,2020
靶点详情
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功能:NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
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亚细胞定位:Secreted. Cytoplasmic vesicle, secretory vesicle, neuronal dense core vesicle.
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蛋白家族:NPY family
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组织特异性:One of the most abundant peptides in the nervous system. Also found in some chromaffin cells of the adrenal medulla.
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数据库链接:
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