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Rat microalbunminuria(MAU/ALB) ELISA kit

  • 中文名称：
    大鼠尿微量白蛋白(MAU/ALB)酶联免疫试剂盒
  • 货号：
    CSB-E12991r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        MAU/ALB（Microalbuminuria/Albumin）是检测肾脏损伤和糖尿病肾病的常用指标。MAU是尿液中微量白蛋白的浓度，高水平提示肾小球滤过膜损伤。ALB是尿液中白蛋白的浓度，高水平提示肾小管受损。对于糖尿病患者来说，MAU/ALB水平的监测可以帮助早期发现并治疗糖尿病肾病。
        j9九游会登录入口首页生物所提供的Rat microalbunminuria(MAU/ALB) ELISA kit属于ELISA检测试剂盒，采用双抗夹心法定量检测大鼠血清、血浆、尿液样本中的MAU/ALB，其灵敏度为0.01 μg/mL，检测范围为0.01 μg/mL-40 μg/mL。
     
  • 别名：
    Alb 1 ELISA Kit; alb ELISA Kit; ALBU_RAT ELISA Kit; Albumin ELISA Kit; Serum albumin ELISA Kit
  • 缩写：
    MAU/ALB
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, urine
  • 检测范围：
    0.01 μg/mL-40 μg/mL
  • 灵敏度：
    0.01 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat MAU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1000 Average % 93  
    Range % 85-107  
    1:2000 Average % 95  
    Range % 87-104  
    1:4000 Average % 89  
    Range % 84-100  
    1:8000 Average % 90  
    Range % 81-102  
  • 回收率：
    The recovery of rat MAU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 88-97  
    EDTA plasma (n=4) 97 90-105  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average Corrected  
    40 2.345 2.412 2.379 2.279  
    10 2.176 2.210 2.193 2.093  
    2.5 1.682 1.657 1.670 1.570  
    0.625 1.237 1.253 1.245 1.145  
    0.156 0.783 0.802 0.793 0.693  
    0.039 0.429 0.436 0.433 0.333  
    0.01 0.272 0.281 0.277 0.177  
    0 0.101 0.098 0.100 　  
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 最新研究进展：
    MAU和ALB是人体中的两种蛋白质，MAU指尿微量白蛋白，ALB指白蛋白，两者都是在肝脏中合成的。目前的研究表明，MAU/ALB比值是评估糖尿病肾病患者肾功能的一个重要指标，其变化可以提前预测肾功能损伤的程度。
  • 功能：
    Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma. Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner. The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood. The rank order of affinity is zinc > calcium > magnesium. Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli. Does not prevent iron uptake by the bacterial siderophore aerobactin.
  • 基因功能参考文献：
    1. Tubular Expression and Urinary Excretion of MMP-9 Protein Were Increased in Association with Albumin Overload in the Diabetic Kidneys. PMID: 28805677
    2. albumin immunoreactivity was newly observed in microglia in the hippocampal CA1 region following 5 min of transient cerebral ischemia. These results indicate that transient ischemia-induced albumin expression in microglia might be associated with ischemia-induced 'delayed neuronal death' in the hippocampal CA1 pyramidal neurons. PMID: 28586018
    3. significant structural changes occur to albumin with glycation, particularly in the FcRn-binding region, which could explain the reduced affinity to FcRn PMID: 26887834
    4. Physiological Levels of Pentraxin 3 and Albumin Attenuate Vascular Endothelial Cell Damage Induced by Histone H3 PMID: 26806786
    5. Megalin/cubilin and lysosome rupture is involved in albumin-triggered tubular injury and tubulointerstitial inflammation. PMID: 26025362
    6. Both insulin and high glucose concentrations enhance the permeability of podocytes to albumin by stimulating oxygen free radical production, primarily by NAD(P)H oxidase-4 (NOX4), and by activating protein kinase G, isoform Ialpha. PMID: 25888796
    7. Overall, rats with albumin deficiency display enhanced glucose tolerance, insulin secretion and gluconeogenic flux. PMID: 22198013
    8. albumin prevents 6-hydroxydopamine-induced loss of tyrosine hydroxylase PMID: 22815976
    9. Data indicate that transcription of Alb is regulated by a complicated system of distant enhancers. PMID: 21858039
    10. This pioneering study determined a higher level of ischemia-modified albumin in a testicular torsion model. PMID: 20452583
    11. Forced expression of PPAR-gamma or C/EBP-alpha in activated pancreatic stellate cells induced albumin expression, thereby reverting cells to the quiescent phenotype. PMID: 19932685
    12. Catalysis of S-nitrosothiols formation by serum albumin: the mechanism and implication in vascular control PMID: 11983891
    13. This study suggests that Aroclor 1254 induces elevated albumin serum levels indirectly through induction of liver-enriched transcription factors, which regulate albumin gene expression PMID: 12051991
    14. an accelerated plasma efflux of albumin contributes to hypoalbuminemia only during the early period of sepsis PMID: 12571074
    15. albumin affects glucose metabolism by impairing insulin-induced insulin receptor substrate (IRS) signaling through a protein kinase C alpha-mediated mechanism PMID: 12970360
    16. Kinetics of albumin production during rat liver development. PMID: 17879097

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    ALB/AFP/VDB family
  • 组织特异性：
    Plasma.
  • 数据库链接：