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Rat matrix metalloproteinase 3,MMP-3 ELISA kit

  • 中文名称：
    大鼠基质金属蛋白酶3(MMP-3)酶联免疫试剂盒
  • 货号：
    CSB-E07410r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat MMP3 ELISA Kit was designed for the quantitative measurement of Rat MMP3 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL .

  • 别名：
    Mmp3 ELISA Kit; Stromelysin-1 ELISA Kit; SL-1 ELISA Kit; EC 3.4.24.17 ELISA Kit; Matrix metalloproteinase-3 ELISA Kit; MMP-3 ELISA Kit; PTR1 protein ELISA Kit; Transin-1 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    1.56 pg/mL-100 pg/mL
  • 灵敏度：
    0.39 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %90
    Range %87-93
    1:200Average %94
    Range %90-98
    1:400Average %95
    Range %91-99
    1:800Average %92
    Range %88-96
  • 回收率：
    The recovery of rat MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8581-89
    EDTA plasma (n=4)9490-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.569 2.547 2.558 2.429
    502.236 2.213 2.225 2.096
    251.868 1.829 1.849 1.720
    12.51.367 1.387 1.377 1.248
    6.250.840 0.889 0.865 0.736
    3.130.377 0.387 0.382 0.253
    1.560.247 0.259 0.253 0.124
    00.128 0.130 0.129 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix. Secreted.
  • 蛋白家族：
    Peptidase M10A family
  • 数据库链接：

    UniGene: Rn.32086