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Rat matrix metalloproteinase 13,MMP-13 ELISA kit

  • 中文名称：
    大鼠基质金属蛋白酶13(MMP-13)酶联免疫试剂盒
  • 货号：
    CSB-E07412r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat MMP13 ELISA Kit was designed for the quantitative measurement of Rat MMP13 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 93.75 pg/mL-6000 pg/mL and the sensitivity is 23.44 pg/mL .

  • 别名：
    Mmp13 ELISA Kit; Collagenase 3 ELISA Kit; EC 3.4.24.- ELISA Kit; Matrix metalloproteinase-13 ELISA Kit; MMP-13 ELISA Kit; UMRCASE ELISA Kit; Fragment ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    93.75 pg/mL-6000 pg/mL
  • 灵敏度：
    23.44 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %83-90
    1:2Average %94
    Range %90-98
    1:4Average %98
    Range %93-104
    1:8Average %102
    Range %98-105
  • 回收率：
    The recovery of rat MMP-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-99
    EDTA plasma (n=4)9085-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    60002.231 2.269 2.250 2.138
    30001.526 1.539 1.533 1.421
    15000.974 0.997 0.986 0.874
    7500.578 0.596 0.587 0.475
    3750.345 0.327 0.336 0.224
    187.50.251 0.241 0.246 0.134
    93.750.185 0.189 0.187 0.075
    00.111 0.113 0.112 　
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.
  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix. Secreted.
  • 蛋白家族：
    Peptidase M10A family
  • 数据库链接：