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Rat matrix metalloproteinase 10,MMP-10 ELISA kit

  • 中文名称：
    大鼠基质金属蛋白酶10(MMP-10)酶联免疫试剂盒
  • 货号：
    CSB-E07414r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat MMP10 ELISA Kit was designed for the quantitative measurement of Rat MMP10 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 80 pg/mL-2000 pg/mL and the sensitivity is 50 pg/mL.

  • 别名：
    Mmp10 ELISA Kit; Stromelysin-2 ELISA Kit; SL-2 ELISA Kit; EC 3.4.24.22 ELISA Kit; Matrix metalloproteinase-10 ELISA Kit; MMP-10 ELISA Kit; Transformation-associated protein 34A ELISA Kit; Transin-2 ELISA Kit
  • 缩写：
    MMP10
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    80 pg/mL-2000 pg/mL
  • 灵敏度：
    50 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 91
    Range % 85-99
    1:2 Average % 92
    Range % 90-103
    1:4 Average % 95
    Range % 87-105
    1:8 Average % 95
    Range % 87-99
  • 回收率：
    The recovery of rat MMP-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 96 88-100
    EDTA plasma (n=4) 95 89-102
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average
    2000 0.172 0.175 0.174
    800 0.376 0.379 0.378
    480 0.554 0.562 0.558
    200 0.882 0.897 0.890
    80 1.493 1.475 1.484
    0 2.404 2.452 2.428
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.
  • 基因功能参考文献：
    1. MMP-10 modulates corneal epithelial wound healing. PMID: 24339723
  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Peptidase M10A family
  • 数据库链接：