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Rat low density lipoprotein receptor,LDLR ELISA Kit

  • 中文名称：
    大鼠低密度脂蛋白受体(LDLR)酶联免疫试剂盒
  • 货号：
    CSB-E13545r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat LDLR ELISA Kit was designed for the quantitative measurement of Rat LDLR protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.

  • 别名：
    Ldlr ELISA Kit; Low-density lipoprotein receptor ELISA Kit; LDL receptor ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    7.8 pg/mL-500 pg/mL
  • 灵敏度：
    1.95 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat LDLR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:200 Average % 85
    Range % 80-89
    1:400 Average % 102
    Range % 98-108
    1:800 Average % 90
    Range % 85-94
    1:1600 Average % 97
    Range % 92-101
  • 回收率：
    The recovery of rat LDLR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 88 84-92
    EDTA plasma (n=4) 93 89-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    500 2.512 2.475 2.494 2.355
    250 2.247 2.196 2.222 2.083
    125 1.803 1.689 1.746 1.607
    62.5 1.189 1.227 1.208 1.069
    31.2 0.687 0.713 0.700 0.561
    15.6 0.415 0.392 0.404 0.265
    7.8 0.298 0.289 0.294 0.155
    0 0.136 0.141 0.139 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits.
  • 基因功能参考文献：
    1. LDLR is a relevant receptor for CNS drug delivery via receptor-mediated transcytosis and that the peptide vectors we developed have the potential to transport drugs PMID: 28108572
    2. Intrauterine growth restriction combined with a maternal high-fat diet increases hepatic cholesterol accumulation via increased LDL cholesterol uptake into the liver with resulting persistent increases in hepatic cholesterol accumulation. PMID: 27401460
    3. Compared with the cholesterol-enriched restructured pork group, silicon-enriched restructured pork rats had lower VLDL compound concentrations and lower VLDL oxidation but greater Ldlr expression. PMID: 26246324
    4. The LDL receptor could be located at once in clathrin-coated pits and caveolae. PMID: 24530906
    5. LDLR mutant rats are a useful novel animal model of hypercholesterolemia and atherosclerosis. PMID: 22293196
    6. A tissue-specific dysregulation of LDLr is demonstrated that is age- and gender-dependent. PMID: 21792919
    7. expression of LDLR was increased in the hippocampus of stroke-prone spontaneously hypertensive rats and was seen in the luminal and abluminal cytoplasmic membranes and vesicular structures of endothelial cells PMID: 20458492
    8. expression pattern of LDL-R in brain microvessels endothelial cells and its modulation by astrocytes PMID: 12095612
    9. cloning and sequencing of the rat LDL receptor 5'-flanking region revealed the presence of several putative cis-acting elements PMID: 16647292
    10. Retina is capable of rapid uptake of circulating LDL via LDLR-mediated process primarily occurring in retinal pigment epithelium and also possibly Muller cells. PMID: 17110914
    11. Caloric restriction affects Ldlr involved in age-related hypercholesterolemia. PMID: 17203467
    12. LDLR-mediated pathway was involved in the endocytosis of Poly(methoxypolyethyleneglycol cyanoacrylate-co-hexadecylcyanoacrylate) (PEG-PHDCA) nanoparticles by RBEC. PMID: 17256088
    13. Modeling of corticosteroid effects on hepatic low-density lipoprotein receptors and plasma lipid dynamics in rats. PMID: 17674160

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Membrane, clathrin-coated pit. Golgi apparatus. Early endosome. Late endosome. Lysosome.
  • 蛋白家族：
    LDLR family
  • 数据库链接：