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  4. Rat leukemia inhibitory factor,LIF ELISA Kit

Rat leukemia inhibitory factor,LIF ELISA Kit

  • 中文名称：
    大鼠白血病抑制因子(LIF)酶联免疫试剂盒
  • 货号：
    CSB-E07432r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat LIF ELISA Kit was designed for the quantitative measurement of Rat LIF protein in serum, plasma, bronchoalveolar lavage fluid, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .

  • 别名：
    LifLeukemia inhibitory factor ELISA Kit; LIF ELISA Kit; Cholinergic neuronal differentiation factor ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, bronchoalveolar lavage fluid, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat LIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %89
    Range %84-95
    1:2Average %93
    Range %89-97
    1:4Average %99
    Range %93-104
    1:8Average %90
    Range %86-98
  • 回收率：
    The recovery of rat LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8782-92
    EDTA plasma (n=4)9589-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.249 2.174 2.212 2.039
    101.583 1.561 1.572 1.399
    51.012 0.952 0.982 0.809
    2.50.599 0.583 0.591 0.418
    1.250.446 0.416 0.431 0.258
    0.6250.320 0.299 0.310 0.137
    0.3120.258 0.245 0.252 0.079
    00.177 0.169 0.173 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    LIF/OSM family
  • 数据库链接：