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  4. Rat hepatocyte growth factor,HGF ELISA kit

Rat hepatocyte growth factor,HGF ELISA kit

  • 中文名称：
    大鼠肝细胞生长因子(HGF)酶联免疫试剂盒
  • 货号：
    CSB-E07346r
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This rat hepatocyte growth factor (HGF) ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of rat HGF in multiple samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of HGF bound in the initial step.

    HGF is synthesized by stromal and mesenchymal cells and functions as a pleiotropic cytokine on cells of mainly epithelial origin. It regulates cell motility and morphogenesis, induces angiogenesis, and promotes cell proliferation and migration by activating a tyrosine kinase signaling cascade after engagement to the proto-oncogenic c-Met receptor. The HGF-Met signaling pathway contributes to the development of epithelial organs in a paracrine manner, exerts regenerative effects on the epithelium, and facilitates the regression of fibrosis in numerous organs. The signaling of HGF through Met is associated with the biology of cancer types, neurons, and immunity. HGF is known to promote tumor progression and inhibit the development of fibrosis after tissue injury.

  • 别名：
    HgfHepatocyte growth factor ELISA Kit; Hepatopoietin-A ELISA Kit; Scatter factor ELISA Kit; SF) [Cleaved into: Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain] ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat HGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %97
    Range %93-103
    1:2Average %94
    Range %91-97
    1:4Average %108
    Range %98-112
    1:8Average %87
    Range %80-92
  • 回收率：
    The recovery of rat HGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9086-94
    EDTA plasma (n=4)9591-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.185 2.284 2.235 2.061
    51.703 1.802 1.753 1.579
    2.51.276 1.295 1.286 1.112
    1.250.903 0.894 0.899 0.725
    0.6250.650 0.641 0.646 0.472
    0.3120.411 0.402 0.407 0.233
    0.1560.319 0.312 0.316 0.142
    00.173 0.174 0.174 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.
  • 基因功能参考文献：
    1. HGF significantly mitigated the severity of pulmonary artery hypertension (PAH) and inhibited inflammation by attenuating the NF-kB signaling in the lungs of PAH rats. PMID: 29442198
    2. Our results suggest that hUCMSCs can promote ovarian expression of HGF, VEGF, and IGF-1 through secreting those cytokines, resulting in improving ovarian reserve function and withstanding ovarian senescence. PMID: 28279229
    3. eIf2alpga expression was upregulated during endoplasmic reticulum stress and interfering eIf2alpha expression proportionately down-regulated HGF expression. PMID: 28844859
    4. VEGF treatment increases expression of HGF in vitro and in vivo and that, based on a series of integrative studies, we suggest that the effects of VEGF are mediated through downstream HGF signaling. PMID: 27036872
    5. Hepatocyte growth factor (Hgf) plays a crucial role in the formation of the muscular diaphragmatic components by regulating the migration of myogenic progenitor cells into the primordial diaphragm. PMID: 27480986
    6. Mesenchymal stem cells restore lung vascular permeability and alleviate lung injury in part by maintaining HGF levels. PMID: 27129877
    7. The mechanism of antifibrogenic action of OBE is hypothesized to proceed via scavenging free radicals and activating liver regeneration by induction of HGF. PMID: 26213907
    8. Data show that secretion of hepatocyte growth factor (HGF) was elevated, tumour necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta1 (TGF-beta1) levels were decreased after infusion of mesenchymal stem cells. PMID: 25736907
    9. HGF induction is a crucial contributor to the acceleration of cell dedifferentiation and growth. PMID: 25793303
    10. HGF was hardly detectable in normal tympanic membrane; however, a significant increase was noted in its expression starting from the third day after injury throughout the follow-up period, with the highest level on day 05. PMID: 25920966
    11. p27 haploinsufficiency protects against heart injury by VEGF/HGF mediated cardioprotection and increased angiogenesis through promoting IKK activation. PMID: 25099287
    12. spleen seemed to exhibit an inhibitory effect on liver regeneration by downregulating HGF and its receptor c-Met in the liver PMID: 25862490
    13. Mesenchymal stromal cells increased serum and tissue HGF levels, Met tubular expression and prevented the suppression of tubular MSP/RON expression. PMID: 25277788
    14. Hepatocyte growth factor upregulates nexilin gene expression in cardiomyocytes via JNK pathway. PMID: 25062485
    15. Studied the preventive and control effect of matrine on transforming growth factor (TGF-beta1) and hepatocyte growth factor (HGF) of liver fibrosis tissue in rats. PMID: 25063067
    16. Transplanted mesenchymal stem cells expressing human HGF combined with G-CSF potentially offer synergistic therapeutic benefit for the treatment of pulmonary arterial hypertension. PMID: 23933910
    17. cytoskeletal rearrangement and cell-cell adhesion, such as through VE-cadherin and ZO-1, are candidate mechanisms for the influence of HGF on the blood-brain barrier PMID: 24370951
    18. these results indicate that HGF activates the cMetAktTGIF signaling pathway, inhibiting CF proliferation and transformation in response to TGFb1 stimulation. PMID: 24840640
    19. Fibroblasts activated by hepatoma cell supernatant express COX-2 and HGF at higher levels. PMID: 24815169
    20. HGF/c-Met signaling pathway plays an essential role in the homing of MSCs towards injured liver triggered by intestinal ischemia-reperfusion, and then mediates MSC-induced liver repair. PMID: 24782653
    21. results indicated that HGF, MMP-9 and TGF-beta1 may participate in the formation and prognosis of hydrocephalus. PMID: 23270462
    22. the results provide evidence that HGF can accelerate reepithelialization in skin wound healing by dedifferentiation of epidermal cells in a manner related to the b1-integrin/ILK pathway. PMID: 24490163
    23. This data indicates that hypoxic modulation of satellite cell-mediated angiogenesis involves a reduction in satellite cell HGF expression. PMID: 24122166
    24. These results strongly suggest that the combined stimulation of IL-6 and glucocorticoids induces the activation of both Reg and HGF genes. PMID: 23954444
    25. HGF is important in the repair of the dentine-pulp complex potentially participating in several aspects of wound healing. PMID: 23273597
    26. HGF is a growth factor playing a key role in islet mass increase and hyperinsulinemia in diet-induced obesity rats; the HGF-Met axis may have a role on insulin signaling in the liver PMID: 23024263
    27. HGF, bFGF and VEGF significantly increased the growth of vascular endothelial cells. PMID: 22361334
    28. The renal fibrosis of CAAN could be protected by isogenic MSC transplantation, probably via upregulation of HGF and downregulation of TGF-beta1. PMID: 22661380
    29. The antifibrotic effects of Astragalus mongholicus may be associated with up-regulation of HGF expression and down-regulation of TGF-beta1 expression in rats with unilateral ureteral obstruction. PMID: 19069655
    30. HGF is a biomarker and mediator in cardioprotection and cardiovascular regeneration [review] PMID: 22318499
    31. The data showed that the activation of hepatocyte growth factor/c-Met signaling is a major pathway involved type II cell proliferation induced by ultrafine carbon black. PMID: 22245998
    32. Upregulation of miRNA-29 by HGF and downregulation by TGF-beta take part in the anti- or profibrogenic response of HSC, respectively PMID: 21931759
    33. in the same stages, hepatocyte growth factor reduces the amount of actin present in the blood testis barrier region, in which occludin levels are highest and modifies the morphology of the actin cytoskeleton network PMID: 21843593
    34. Hepatocyte growth factor-induced amelioration in renal interstitial fibrosis is associated with reduced expression of alpha-smooth muscle actin and transforming growth factor-beta1 PMID: 22165288
    35. HGF and VEGF participate in the pathological injury and repair of cerebral tissue in rats with chronic hydrocephalus after subarachnoid hemorrhage. PMID: 21643626
    36. Both HGF and glial cell line-derived neurotrophic factor (GDNF) have significant neurotrophic effects, but only HGF can promote neurogenesis, angiogenesis, and synaptogenesis and inhibit fibrosis in brains after transient middle cerebral artery occlusion. PMID: 20963849
    37. Ezrin, HGF and C-met may have positive effects on the carcinogenesis of rat pancreas PMID: 21134835
    38. VEGF and TGFbeta might modulate HGF signaling to influence route selection during cancer progression PMID: 21075102
    39. HGF expression following sciatic nerve ligation was found decreased in ipsilateral dorsal root ganglions from day 3 to day 14. AND no significant change in HGF expression was detected in the lumbar spinal cords. PMID: 20814222
    40. protective effects of GDNF and HGF on brain ischemia were associated with both antiapoptotic and antiautophagic effects; maybe two types of cell death can occur in the same cell at the same time, and GDNF and HGF are capable of ameliorating both pathways PMID: 20175208
    41. HGF influences the metabolic activity of isolated Leydig cells (ie, uPA and MMP 2 secretion) and increases TGF-beta activity PMID: 19834131
    42. HGF counteracted the effect of high glucose on protein kinase A and protein kinase G activity. Thus, inhibition of PKA and activation of PKG are involved in the antioxidant role of HGF. PMID: 20466058
    43. Valsartan alleviates BLM-induced pulmonary fibrosis in rats, possibly by inhibiting the expression of TGF-beta and stimulating the expression of HGF in lung tissues. PMID: 16115399
    44. HGF may play an important role in the early stages of dental pulp repair. PMID: 17294746
    45. HGF-induced EPC proliferation is mediated partly via activation of STIM1 PMID: 20404049
    46. HGF expression was significantly increased in control rats treated with Astragalus mongholicus compared to rats with unilateral ureteral obstruction. PMID: 19292056
    47. Over-expression of HGF in vascular smooth muscle cells can be helpful for promoting endothelial stem cells differentiation, increasing their migration and proliferation PMID: 19095317
    48. HGF is expressed in primary sensory neurons from dorsal root ganglia and spinal cord, and may be involved in sensory transmission. PMID: 11744165
    49. Differential mitogenic effects of single chain hepatocyte growth factor (HGF)/scatter factor and HGF/NK1 following cleavage by factor Xa PMID: 11832492
    50. results suggest that hepatocellular hypoxia causes inhibition of HGF-mediated proliferation PMID: 11839582

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  • 蛋白家族：
    Peptidase S1 family, Plasminogen subfamily
  • 数据库链接：