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  4. Rat heat shock protein,HSP47 ELISA Kit

Rat heat shock protein,HSP47 ELISA Kit

  • 中文名称：
    大鼠热休克蛋白47(HSP47)酶联免疫试剂盒
  • 货号：
    CSB-E11328r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The Rat HSP47/Serpin H1 ELISA Kit is a high-quality research tool designed for the quantitative detection of Serpin H1 in rat serum, plasma, tissue homogenates, and cell lysates. This kit is specifically designed for use in neuroscience research and can detect levels of Serpin H1 as low as 0.039 ng/mL with a detection range of 0.156 ng/mL to 10 ng/mL.

    The assay principle is based on the sandwich technique, in which the capture antibody is pre-coated on a microplate and the detection antibody is labeled with biotin. Upon binding to the target analyte, the complex is captured by streptavidin-HRP conjugate and the substrate TMB is added to produce a colored reaction product that is quantified by measuring the absorbance at 450 nm.

    With an assay time of 1-5 hours, a sample volume of 50-100 µl, and a detection wavelength of 450 nm, the HSP47 ELISA kit provides accurate and reliable results for your research needs. The kit includes all necessary components for the assay, such as microplate wells, wash buffer, detection antibody, and standards.

  • 别名：
    Serpinh1 ELISA Kit; Cbp1 ELISA Kit; Hsp47 ELISA Kit; Serpin H1 ELISA Kit; 47 kDa heat shock protein ELISA Kit; Collagen-binding protein ELISA Kit; Colligin ELISA Kit; GP46 ELISA Kit
  • 缩写：
    SERPINH1
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat HSP47 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %87
    Range %81-93
    1:2Average %102
    Range %98-105
    1:4Average %92
    Range %88-96
    1:8Average %95
    Range %91-99
  • 回收率：
    The recovery of rat HSP47 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9289-96
    EDTA plasma (n=4)8783-92
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.311 2.507 2.409 2.261
    51.740 1.645 1.693 1.545
    2.51.087 1.124 1.106 0.958
    1.250.664 0.652 0.658 0.510
    0.6250.420 0.433 0.427 0.279
    0.3120.339 0.327 0.333 0.185
    0.1560.226 0.230 0.228 0.080
    00.147 0.148 0.148
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
  • 基因功能参考文献：
    1. The results suggest that Hsp47 turnover may increase during heat stress or that Hsp47 consumption exceeds its production. PMID: 25526199
    2. miR-29b down-regulates heat shock protein 47 and LOX expression. PMID: 24650661
    3. HSP47 specifically modulates TGF-beta1-induced extracellular matrix protein synthesis in HK-2 cells PMID: 22718885
    4. Heat shock protein 47 (HSP47) antisense oligonucleotides reduce cardiac remodeling and improve cardiac function in a rat model of myocardial infarction. PMID: 21412710
    5. Involvement of HSP47 in progression of peritoneal fibrosis. Inhibition of HSP47 expression might merit further clinical investigation for treatment of peritoneal fibrosis in continuous ambulatory peritoneal dialysis. PMID: 12911538
    6. Microgravity differentially modulated the expression of the molecular chaperone HSP47 in osteoblasts PMID: 15033773
    7. expression of HSP47, a collagen-specific molecular chaperone, responds to gravitational changes, including microgravity and hypergravity in vitro and in vivo PMID: 17054723

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  • 亚细胞定位：
    Endoplasmic reticulum lumen.
  • 蛋白家族：
    Serpin family
  • 数据库链接：