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  4. Rat guanylate cyclase activator 2B (uroguanylin) (GUCA2B) ELISA kit

Rat guanylate cyclase activator 2B (uroguanylin) (GUCA2B) ELISA kit

  • 中文名称：
    大鼠鸟苷酸环化酶激活剂2B（尿鸟苷素）(GUCA2B)酶联免疫试剂盒
  • 货号：
    CSB-EL010048RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat GUCA2B ELISA Kit was designed for the quantitative measurement of Rat GUCA2B protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.

  • 别名：
    Guca2b ELISA Kit; Guanylate cyclase activator 2B [Cleaved into: Uroguanylin ELISA Kit; UGN)] ELISA Kit
  • 缩写：
    GUCA2B
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    7.8 pg/mL-500 pg/mL
  • 灵敏度：
    1.95 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat GUCA2B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 97
    Range % 88-102
    1:2 Average % 92
    Range % 85-96
    1:4 Average % 90
    Range % 85-94
    1:8 Average % 91
    Range % 84-95
  • 回收率：
    The recovery of rat GUCA2B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 88 81-92
    EDTA plasma (n=4) 104 92-107
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    500 1.997 1.943 1.970 1.831
    250 1.508 1.476 1.492 1.353
    125 1.002 0.987 0.995 0.856
    62.5 0.572 0.565 0.569 0.430
    31.25 0.428 0.420 0.424 0.285
    15.6 0.289 0.276 0.283 0.144
    7.8 0.204 0.198 0.201 0.062
    0 0.141 0.137 0.139 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Endogenous activator of intestinal guanylate cyclase. It stimulates this enzyme through the same receptor binding region as the heat-stable enterotoxins. May be a potent physiological regulator of intestinal fluid and electrolyte transport. May be an autocrine/paracrine regulator of intestinal salt and water transport.
  • 基因功能参考文献：
    1. our data suggest that the inhibitory effect of UGN on NHE3 transport activity in proximal tubule is mediated by activation of both cGMP/PKG and cAMP/PKA signaling pathways PMID: 22952280
    2. Uroguanylin is delivered to the kidney as an unprocessed propeptide. PMID: 18499760
    3. To respond to proUGn released from the gut, any target organ must use a local mechanism for production of active peptide. PMID: 18499761
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Guanylin family
  • 组织特异性：
    Expressed not only in the gastrointestinal tract but also in the lung, pancreas and kidney.
  • 数据库链接：