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  4. Rat fructosamin (FRA) ELISA kit

Rat fructosamin (FRA) ELISA kit

  • 中文名称：
    大鼠果糖胺(FRA)酶联免疫试剂盒
  • 货号：
    CSB-E13451r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat FRA ELISA Kit was designed for the quantitative measurement of Rat FRA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 nmol/mL-2000 nmol/mL and the sensitivity is 7.81 nmol/mL.

  • 缩写：
    FRA
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 nmol/mL-2000 nmol/mL
  • 灵敏度：
    7.81 nmol/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat FRA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %103
    Range %99-108
    1:2Average %98
    Range %94-102
    1:4Average %93
    Range %89-97
    1:8Average %90
    Range %86-98
  • 回收率：
    The recovery of rat FRA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9590-100
    EDTA plasma (n=4)8984-95
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    nmol/mlOD1OD2AverageCorrected
    20002.362 2.458 2.410 2.229
    10001.943 1.831 1.887 1.706
    5001.206 1.241 1.224 1.043
    2500.754 0.767 0.761 0.580
    1250.395 0.413 0.404 0.223
    62.50.318 0.335 0.327 0.146
    31.250.221 0.234 0.228 0.047
    00.179 0.182 0.181
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Good day. May I ask a question about the kit, CSB-E13451r?
What is the standard? Fructosamines are compounds that result from glycation reactions between a sugar (such as fructose or glucose) and a primary amine.
How does this kit measure every type of fructosamine since many different proteins can be modified in this way?
Thank you for your help with my question.

A:
Thanks for your inquiry.
CSB-E13451r
The standard in the kit is modified NS0-derived recombinant protein.
As you mentioned: Fructosamines are compounds that result from glycation reactions between a sugar (such as fructose or glucose) and a primary amine. We have screened out specific raw materials of antibodies( mouse monoclonal antibody and goat polyclonal antibody). In theory, if the type of fructosamine has the antibody's specific recognition sites, then it can be detected out.
Pls let me know if you have any further questions. Thank you.