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  4. Rat fatty acid synthase (FAS) ELISA kit

Rat fatty acid synthase (FAS) ELISA kit

  • 中文名称：
    大鼠脂肪酸合成酶(FAS)酶联免疫试剂盒
  • 货号：
    CSB-E16440r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat FASN ELISA Kit was designed for the quantitative measurement of Rat FASN protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

  • 别名：
    FasnFatty acid synthase ELISA kit; EC 2.3.1.85) [Includes: [Acyl-carrier-protein] S-acetyltransferase ELISA kit; EC 2.3.1.38); [Acyl-carrier-protein] S-malonyltransferase ELISA kit; EC 2.3.1.39); 3-oxoacyl-[acyl-carrier-protein] synthase ELISA kit; EC 2.3.1.41); 3-oxoacyl-[acyl-carrier-protein] reductase ELISA kit; EC 1.1.1.100); 3-hydroxyacyl-[acyl-carrier-protein] dehydratase ELISA kit; EC 4.2.1.59); Enoyl-[acyl-carrier-protein] reductase ELISA kit; EC 1.3.1.39); Oleoyl-[acyl-carrier-protein] hydrolase ELISA kit; EC 3.1.2.14)] ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.56 ng/mL-100 ng/mL
  • 灵敏度：
    0.39 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat FAS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %90
    Range %85-94
    1:2Average %92
    Range %88-99
    1:4Average %88
    Range %84-92
    1:8Average %95
    Range %90-100
  • 回收率：
    The recovery of rat FAS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10096-108
    EDTA plasma (n=4)9790-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1OD2AverageCorrected
    1002.572 2.427 2.500 2.371
    501.837 1.902 1.870 1.741
    251.353 1.308 1.331 1.202
    12.50.775 0.819 0.797 0.668
    6.250.512 0.516 0.514 0.385
    3.120.305 0.294 0.300 0.171
    1.560.245 0.251 0.248 0.119
    00.131 0.127 0.129
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.
  • 基因功能参考文献：
    1. Calorie restriction during pregnancy modified the hepatic Fasn mRNA transcript level and altered the blood cholesterol concentrations in dams, but there were no such effects in their four-week-old offspring. PMID: 28810876
    2. The involvement of SREBP-1c and ChREBP in FASN promoter histone modification.Histone acetylation affects FASN transcription by influencing ChREBP-binding carbohydrate-responsive elements. PMID: 28027934
    3. This study showed that an associated inhibition of peroxisomal proliferator activated receptor gamma coactivator 1alpha (PGC1alpha), increased fatty acid synthase (FASN) expression in the liver, and reduced adipocyte triglyceride lipase (ATGL) expression. PMID: 26287659
    4. Data suggest expression of Fas in liver can be regulated by dietary components; dietary fructose up-regulates expression of Fas; supplementation with citrulline prevents up-regulation of Fas, insulin resistance, and nonalcoholic fatty liver disease. PMID: 26246323
    5. Results show that genes with the highest expression in the mammary gland, liver and adipose tissue were Elovl5, Fads2, and Fasn, respectively, in a low-lipid diet than in adequate-lipid diet. PMID: 25046614
    6. Epididymal adipose tissue expression of the fatty acid synthase (FASN) gene and NADH dehydrogenase (ubiquinone) 1beta-subcomplex 6 (NDUFB6) were significantly reduced in the high fat diet group PMID: 20729114
    7. suppression of promoter by polyunsaturated fatty acids PMID: 11971939
    8. Transcription factors act on the promoter of the rat fatty acid synthase gene [review] PMID: 12440974
    9. hepatic gene expression of acetyl-coenzyme A carboxylase and fatty acid synthase was increased 2-fold in late low protein and low protein offspring PMID: 12634446
    10. glycerol-3-phosphate dehydrogenase, fatty acid synthase, and acetyl-coa carboxylase mRNA levels are upregulated by glucose, which requires glutamine, and glucosamine PMID: 12759350
    11. Downregulated by rosiglitazone. PMID: 15887043
    12. Prolactin represses expression of transfected rat FAS in mouse adipocytes through STAT5A binding to the -908 to -893 site. PMID: 15983196
    13. Insulin acutely reduces hepatic FAS activity by inducing phosphorylation of the carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) and its interaction with FAS. PMID: 16054098
    14. These results showed that increases in SREBP-1c were neither necessary nor sufficient for Glucokinase induction in hepatocytes, while at the same time they underscored the role of SREBP-1c as a key regulator of Fatty acid synthase. PMID: 16834571
    15. polyunsaturated fatty acids decrease in vivo binding of NF-Y and SREBP-1c to the proximal promoter of the hepatic Fasn gene PMID: 17313375
    16. Insulin, glucose and AICAR (an activator of AMP-activated protein kinase) affected changes in hypothalamic FAS mRNA, which may be regulated via the SREBP1c dependent or independent pathway. PMID: 17709201
    17. Normal flux through Fasn is not required for glucose-stimulated insulin secretion. PMID: 17823126
    18. Findings establish that sequence elements previously assigned to a central structural core region of the type I fatty acid synthases and some modular polyketide synthase counterparts play an essential catalytic role as part of the dehydratase domain. PMID: 18096506
    19. Dietary soy protein, by decreasing circulating insulin levels and colon FASN expression, attenuates insulin-induced DNA damage and FASN-mediated anti-apoptosis during carcinogenesis. PMID: 18239060
    20. There were reduced nuclear levels of the lipogenic transcription factor sterol regulatory element binding protein-1 in MCD-fed mice, but no consistent reduction in fatty acid synthesis genes (acetyl-CoA carboxylase and fatty acid synthase). PMID: 18280001
    21. Results describe the inhibition of fatty acid synthase by flavonoids extracted from Pangdahai (Sterculia). PMID: 18380011

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  • 亚细胞定位：
    Cytoplasm. Melanosome.
  • 数据库链接：

    KEGG: rno:50671

    STRING: 10116.ENSRNOP00000064445

    UniGene: Rn.9486