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  4. Rat eosinophil cationic protein,ECP ELISA Kit

Rat eosinophil cationic protein,ECP ELISA Kit

  • 中文名称：
    大鼠嗜酸性粒细胞阳离子蛋白(ECP)酶联免疫试剂盒
  • 货号：
    CSB-E11238r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat RNASE3 ELISA Kit was designed for the quantitative measurement of Rat RNASE3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

  • 别名：
    Rnase3 ELISA Kit; Ear11 ELISA Kit; Rns3 ELISA Kit; Eosinophil cationic protein ELISA Kit; ECP ELISA Kit; EC 3.1.27.- ELISA Kit; Eosinophil secondary granule ribonuclease 11 ELISA Kit; EAR-11 ELISA Kit; Ribonuclease 3 ELISA Kit; RNase 3 ELISA Kit
  • 缩写：
    RNASE3
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat ECP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 95
    Range % 90-100
    1:2 Average % 101
    Range % 96-107
    1:4 Average % 92
    Range % 88-96
    1:8 Average % 89
    Range % 84-93
  • 回收率：
    The recovery of rat ECP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 94 90-98
    EDTA plasma (n=4) 87 82-91
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    4000 2.651 2.529 2.590 2.382
    2000 2.004 1.903 1.954 1.746
    1000 1.310 1.385 1.348 1.140
    500 0.829 0.851 0.840 0.632
    250 0.492 0.476 0.484 0.276
    125 0.347 0.337 0.342 0.134
    62.5 0.285 0.294 0.290 0.082
    0 0.203 0.212 0.208 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Cytotoxin and helminthotoxin with ribonuclease activity. Possesses a wide variety of biological activities.
  • 亚细胞定位：
    Cytoplasmic granule.
  • 蛋白家族：
    Pancreatic ribonuclease family
  • 数据库链接：