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  4. Rat cyclophilin A,CyPA ELISA Kit

Rat cyclophilin A,CyPA ELISA Kit

  • 中文名称：
    大鼠嗜环蛋白/亲环素A(CyPA)酶联免疫试剂盒
  • 货号：
    CSB-E14928r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat PPIA ELISA Kit was designed for the quantitative measurement of Rat PPIA protein in serum, plasma, cell lysates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL.

  • 别名：
    Ppia ELISA Kit; Peptidyl-prolyl cis-trans isomerase A ELISA Kit; PPIase A ELISA Kit; EC 5.2.1.8 ELISA Kit; Cyclophilin A ELISA Kit; Cyclosporin A-binding protein ELISA Kit; Rotamase A ELISA Kit; p1B15 ELISA Kit; p31) [Cleaved into: Peptidyl-prolyl cis-trans isomerase A ELISA Kit; N-terminally processed] ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell lysates, tissue homogenates
  • 检测范围：
    125 pg/mL-8000 pg/mL
  • 灵敏度：
    31.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CyPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %103
    Range %101-105
    1:2Average %96
    Range %93-98
    1:4Average %89
    Range %84-94
    1:8Average %98
    Range %96-100
  • 回收率：
    The recovery of rat CyPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 106103-109
    EDTA plasma (n=4)9286-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    80002.788 2.595 2.692 2.509
    40002.012 1.987 2.000 1.817
    20001.366 1.323 1.345 1.162
    10000.789 0.790 0.790 0.607
    5000.512 0.526 0.519 0.336
    2500.389 0.382 0.386 0.203
    1250.254 0.264 0.259 0.076
    00.180 0.186 0.183
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Exerts a strong chemotactic effect on leukocytes partly through activation of one of its membrane receptors BSG/CD147, initiating a signaling cascade that culminates in MAPK/ERK activation. Activates endothelial cells (ECs) in a proinflammatory manner by stimulating activation of NF-kappa-B and ERK, JNK and p38 MAP-kinases and by inducing expression of adhesion molecules including SELE and VCAM1. Induces apoptosis in ECs by promoting the FOXO1-dependent expression of CCL2 and BCL2L11 which are involved in EC chemotaxis and apoptosis. In response to oxidative stress, initiates proapoptotic and antiapoptotic signaling in ECs via activation of NF-kappa-B and AKT1 and up-regulation of antiapoptotic protein BCL2. Negatively regulates MAP3K5/ASK1 kinase activity, autophosphorylation and oxidative stress-induced apoptosis mediated by MAP3K5/ASK1. Necessary for the assembly of TARDBP in heterogeneous nuclear ribonucleoprotein (hnRNP) complexes and regulates TARDBP binding to RNA UG repeats and TARDBP-dependent expression of HDAC6, ATG7 and VCP which are involved in clearance of protein aggregates. Plays an important role in platelet activation and aggregation. Regulates calcium mobilization and integrin ITGA2B:ITGB3 bidirectional signaling via increased ROS production as well as by facilitating the interaction between integrin and the cell cytoskeleton. Binds heparan sulfate glycosaminoglycans.
  • 亚细胞定位：
    Cytoplasm. Secreted. Nucleus.
  • 蛋白家族：
    Cyclophilin-type PPIase family, PPIase A subfamily
  • 数据库链接：