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  4. Rat apolipoprotein H (Apo-H) ELISA Kit

Rat apolipoprotein H (Apo-H) ELISA Kit

  • 中文名称：
    大鼠载脂蛋白H(Apo-H)酶联免疫试剂盒
  • 货号：
    CSB-E10073r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    The Rat apolipoprotein H (Apo-H) ELISA Kit is the reliable and accurate solution for detecting and quantifying Beta-2-glycoprotein 1, also known as Apolipoprotein H (Apo-H) or Beta-2-glycoprotein I (B2GPI), in rat serum, plasma, and tissue homogenates.

    With its detection range of 28 ng/mL-1800 ng/mL and a sensitivity of 7 ng/mL, this ELISA kit allows for precise measurements of Apo-H levels in a variety of sample types. The assay time is 1-5 hours, and the sample volume required is only 50-100 µL, making it a convenient and efficient choice for researchers in the cardiovascular field.

    The Rat apolipoprotein H (Apo-H) ELISA Kit follows a sandwich assay principle, where the sample is captured by an immobilized antibody specific to rat Apo-H and then detected by a biotinylated antibody. The amount of detected signal is proportional to the concentration of Apo-H in the sample, which is measured at a detection wavelength of 450 nm. The high sensitivity, wide detection range, and easy-to-use protocol make this kit an ideal choice for researchers in the area.

  • 别名：
    ApohBeta-2-glycoprotein 1 ELISA kit; Apolipoprotein H ELISA kit; Apo-H ELISA kit; Beta-2-glycoprotein I ELISA kit; B2GPI ELISA kit; Beta(2)GPI ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    28 ng/mL-1800 ng/mL
  • 灵敏度：
    7 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Apo-H in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:200Average %91
    Range %86-95
    1:400Average %102
    Range %97-107
    1:800Average %91
    Range %85-97
    1:1600Average %97
    Range %91-103
  • 回收率：
    The recovery of rat Apo-H spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-98
    EDTA plasma (n=4)107101-110
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    18002.655 2.532 2.594 2.464
    9002.164 2.117 2.141 2.011
    4501.597 1.544 1.571 1.441
    2250.845 0.828 0.837 0.707
    1120.501 0.511 0.506 0.376
    560.307 0.300 0.304 0.174
    280.234 0.222 0.228 0.098
    00.131 0.129 0.130
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.
  • 亚细胞定位：
    Secreted.
  • 组织特异性：
    Expressed by the liver and secreted in plasma.
  • 数据库链接：

    UniGene: Rn.1824