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  4. Rat angiotensin 1-7 (Ang1-7) ELISA kit

Rat angiotensin 1-7 (Ang1-7) ELISA kit

  • 中文名称：
    大鼠血管紧张素1-7(Ang1-7)酶联免疫试剂盒
  • 货号：
    CSB-E14241r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat Ang1-7 ELISA Kit was designed for the quantitative measurement of Rat Ang1-7 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.68 pg/mL.

  • 缩写：
    Ang1-7
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    18.75 pg/mL-1200 pg/mL
  • 灵敏度：
    4.68 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %94
    Range %80-105
    1:2Average %96
    Range %85-104
    1:4Average %102
    Range %92-114
    1:8Average %99
    Range %80-110
  • 回收率：
    The recovery of rat Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9683-112
    EDTA plasma (n=4)9590-102
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    12003.121 3.129 3.125 2.986
    6002.532 2.522 2.527 2.388
    3001.739 1.765 1.752 1.613
    1501.050 1.041 1.046 0.907
    750.623 0.642 0.633 0.494
    37.50.435 0.447 0.441 0.302
    18.750.265 0.254 0.260 0.121
    00.135 0.143 0.139 　
  • 数据处理：
  • 货期：
    3-5 working days

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问答及客户评论

■ 常见问题解答
Q:

What are host animals of antibodies in the kit? Are the antibodies affinity purified?

A:
Thanks for your inquiry.Capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody. It is affinity purified. The standard is synthetic peptides. Pls let me know if you have any further questions. Thank you.