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Rat alpha-1-microglobulin/bikunin precursor (AMBP) ELISA kit

  • 中文名称:
    大鼠α1微球蛋白/bikunin蛋白前体(AMBP)酶联免疫试剂盒
  • 货号:
    CSB-EL001654RA
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:
        Alpha-1-microglobulin/bikunin precursor (AMBP)是一种蛋白质,它由alpha-1-microglobulin (A1M)和bikunin(一种Kunitz型蛋白酶抑制剂)组成的前体蛋白。A1M是一种广泛分布的蛋白质,存在于许多组织和体液中,具有多种生物学功能,如抗氧化作用、免疫调节和铁离子绑定等。Bikunin则是一种强力的抗炎剂和抗凝剂。AMBP的生物学功能在很大程度上由A1M和bikunin的功能共同作用决定。AMBP在体内被认为参与多种生理和病理过程,包括肾脏疾病、糖尿病、心血管疾病等。因此,AMBP在临床上也作为一种生物标志物,用于评估这些疾病的风险和进展。

        j9九游会登录入口首页生物所提供的Rat alpha-1-microglobulin/bikunin precursor (AMBP) ELISA kit属于ELISA检测试剂盒,采用竞争法定量检测大鼠血清、血浆、组织匀浆样本中的AMBP,其灵敏度为0.649 ng/mL,检测范围为1.563 ng/mL-100 ng/mL。
  • 别名:
    Ambp ELISA Kit; ItilProtein AMBP [Cleaved into: Alpha-1-microglobulin; Inter-alpha-trypsin inhibitor light chain ELISA Kit; ITI-LC ELISA Kit; Bikunin ELISA Kit; HI-30); Trypstatin] ELISA Kit
  • 缩写:
    AMBP
  • Uniprot No.:
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    1.563 ng/mL-100 ng/mL
  • 灵敏度:
    0.649 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cancer
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat AMBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
      Sample Serum(n=4)    
    1:100 Average % 105    
    Range % 99-109    
    1:200 Average % 96    
    Range % 92-99    
    1:400 Average % 95    
    Range % 90-98    
    1:800 Average % 89    
    Range % 84-94    
  • 回收率:
    The recovery of rat AMBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 91 87-95    
    EDTA plasma (n=4) 99 99-104    
                   
                   
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average      
    100 0.116 0.124 0.120      
    50 0.231 0.245 0.238      
    25 0.478 0.498 0.488      
    12.5 1.019 1.025 1.022      
    6.25 1.426 1.367 1.397      
    3.125 2.159 2.168 2.164      
    1.563 2.466 2.544 2.505      
    0 2.845 2.654 2.750      
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

靶点详情

  • 最新研究进展:
        AMBP(α1酸性糖蛋白)是一种由肝脏合成的糖蛋白,具有多种生物学功能,包括与免疫系统、炎症反应和病原体识别相关。最近的研究表明,AMBP在多种疾病中发挥着重要作用,例如肾脏疾病、肝病、癌症、糖尿病、心血管疾病等。以下是AMBP的最新研究进展:
        肾脏疾病:AMBP被认为是肾脏疾病的标志物之一,其水平与肾小球滤过率、尿蛋白和炎症反应相关。最近的研究还发现,AMBP可以作为肾小球肾炎的标志物,有助于诊断和监测该疾病的进展。
        癌症:AMBP的水平在多种癌症中明显升高,包括肝癌、胃癌、肺癌等。最近的研究表明,AMBP可能参与了肿瘤的发生、发展和转移,并且可以作为癌症的潜在治疗靶点。
        免疫系统:AMBP可以通过多种方式参与免疫系统调节,包括识别和清除病原体、调节T细胞和B细胞功能、参与炎症反应等。最近的研究表明,AMBP在免疫系统调节中的作用可能是通过与其他分子相互作用,如IgG、Toll样受体(TLR)等。
  • 功能:
    Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.; Trypstatin is a trypsin inhibitor. It inhibits blood coagulation factor Xa and tryptase about 100-fold more rapidly than porcine pancreatic trypsin and chymase.
  • 亚细胞定位:
    Secreted.
  • 蛋白家族:
    Calycin superfamily, Lipocalin family
  • 组织特异性:
    Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine. Trypstatin is present in mast cell gra
  • 数据库链接: