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  4. Rat aldehyde dehydrogenase,ALDH ELISA Kit

Rat aldehyde dehydrogenase,ALDH ELISA Kit

  • 中文名称：
    大鼠乙醛脱氢酶(ALDH)酶联免疫试剂盒
  • 货号：
    CSB-E11179r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat aldehyde dehydrogenase (ALDH) ELISA Kit is designed to measure the levels of Aldehyde dehydrogenase in samples from Rattus norvegicus (Rat). This quantitative assay uses a sandwich ELISA method, providing reliable and accurate measurements of ALDH in serum, plasma, and tissue homogenates.

    With a detection range of 0.312 ng/mL to 20 ng/mL and a sensitivity of 0.078 ng/mL, this Elisa kit enables precise and accurate measurements of ALDH levels in rat samples. The assay time ranges from 1 to 5 hours, requiring only 50-100ul of sample volume. The detection wavelength is at 450 nm.

    The Rat aldehyde dehydrogenase (ALDH) ELISA Kit is ideal for researchers in the signal transduction research area, looking to study the role of ALDH in the metabolism of toxic aldehydes, oxidative stress, and cellular signaling. The kit provides an efficient, accurate and reproducible method for the detection and measurement of ALDH levels in rat samples.

  • 别名：
    Aldh2 ELISA Kit; Aldehyde dehydrogenase ELISA Kit; mitochondrial ELISA Kit; EC 1.2.1.3 ELISA Kit; ALDH class 2 ELISA Kit; ALDH-E2 ELISA Kit; ALDH1 ELISA Kit
  • 缩写：
    ALDH2
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat ALDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %100
    Range %91-105
    1:2Average %98
    Range %94-103
    1:4Average %96
    Range %90-100
    1:8Average %98
    Range %95-102
  • 回收率：
    The recovery of rat ALDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9086-95
    EDTA plasma (n=4)9187-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.764 2.753 2.759 2.560
    102.159 2.042 2.101 1.902
    51.529 1.512 1.521 1.322
    2.51.045 1.018 1.032 0.833
    1.250.682 0.656 0.669 0.470
    0.6250.423 0.410 0.417 0.218
    0.3120.312 0.303 0.308 0.109
    00.201 0.197 0.199 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 基因功能参考文献：
    1. ALDH2 inhibition protects against hepatic ischemia-reperfusion injury in rats via alleviation of oxidative stress. PMID: 29589772
    2. findings suggested that ALDH2 is expressed in neonate rat cardiac fibroblasts; activation of ALDH2 decreases the HG-induced apoptosis and fibrosis through inhibition of oxidative stress. PMID: 29129988
    3. ALDH2 O-GlcNAc modification is associated with hyperglycemic exacerbation during myocardial ischemia/reperfusion injury. PMID: 28038474
    4. Chronic hyperglycemia reduces ALDH2 activity, leading to mitochondrial respiratory dysfunction and consequently cardiac damage and dysfunction. PMID: 27736868
    5. Mitochondrial aldehyde dehydrogenase activation by alda-1 led to a significant attenuation of depressive- and anxiety-like behaviors in the prenatally stressed rats. PMID: 26254233
    6. High concentrations of ethanol aggravate cerebral injury by inhibiting of aldehyde dehydrogenase 2 and inducing excess accumulation of aldehydes PMID: 26172086
    7. The administration of the ALDH2-coding vector to the ventral tegmental area leading to an increased ability to degrade acetaldehyde greatly reduces chronic alcohol consumption and post-deprivation binge drinking for prolonged periods. PMID: 24571199
    8. Aberrant hypermethylation of CpG sites in ALDH2 promoter upstream sequence is associated with myocardial ischemia injury. PMID: 25629048
    9. ALDH2 is inhibition of MPTP opening and iNOS expression in cardiac function in response to treatment with Alda1, a specific activator of ALDH2. PMID: 25351957
    10. ALDH2 may be at least in part involved in nitrite-mediated hypotensive effects and nitrite catalysis in many organs of normoxic rats. PMID: 24164360
    11. Selective activation of mitochondrial aldehyde dehydrogenase 2 is sufficient to improve the heart failure outcome PMID: 24817685
    12. the mitochondrial enzyme ALDH2 regulates nociception PMID: 25163478
    13. Ethanol was used induce ALDH2 activity, which decreased myocardial injury in diabetic rats. PMID: 23500772
    14. In diabetic rats, enhanced ALDH2 expression can offer mycardial protection possibly by suppressing cell apoptosis. PMID: 22445980
    15. In diabetic rats, the expression of ALDH2 was decreased when the heart was subjected to ischemia/reperfusion. PMID: 22737913
    16. ALDH2 activation substantially reduces hypoxia-induced norepinephrine release during myocardial ischemia. PMID: 22761303
    17. The protective role of ALDH2 against ER stress-induced cell death was probably mediated by Akt via a p47(phox) NADPH oxidase-dependent manner. These findings indicate the critical role of ALDH2 in the pathogenesis of ER stress in heart disease. PMID: 22430940
    18. Changes in the GSH and GSH enzymatic system, and in the ADH3 and ALDH2 retinal expression and distribution might be crucial in assessing the intrinsic mechanisms of LPO-mediated retinopathies. PMID: 22117533
    19. study provides evidence that the simultaneous increase of liver ADH and a reduction of ALDH activity by gene transfer could constitute a potential therapeutic strategy for the treatment of alcoholism. PMID: 22214999
    20. our results suggested that ALDH2 might play a vital role in protecting antimycin A-induced oxidative stress and apoptosis in cardiomyocytes PMID: 21123025
    21. A decrease was observed in both ALDH2 protein expression (80% in tolerant veins and 30% in tolerant arteries after 48 h exposure to GTN) and aortic ALDH activity. PMID: 21506955
    22. Overexpressed ALDH2 gene may moderate 4-hydroxynonenal-induced neuronal death by regulating caspase-3 protein and reactive oxygen species level in cultured hippocampal neurons. PMID: 20361289
    23. the effects of Mg(2+) on the ALDH2-NADH binding characteristics and enzyme catalysis PMID: 21276780
    24. Data suggest that ALDH2 inhibition may be associated with LV reduced contractility, and mitochondrial impairment aggravated by ALDH2 inhibition might reflect an underlying mechanism which causes cardiac dysfunction in diabetic rats. PMID: 20957334
    25. Ethanol postconditioning upregulated mitochondrial ALDH2 mRNA expression in isolated rat heart. PMID: 21166048
    26. this finding raises the possibility that ALDH2 as the transcriptional regulator might function as a direct sensor of the cellular metabolic state for regulating gene expression at general transcription level. PMID: 21130747
    27. Inhibition of this enzyme is effective in suppressing cocaine-seeking behavior, and may beo of therapeutic value for treating human cocaine addiction and preventing relapse. PMID: 20729865
    28. ALDH2 plays an important role in the bioactivation of glyceryl trinitrate and nitrite in the pulmonary and systemic vascular beds PMID: 20543077
    29. The line difference in AcH aversion is associated more with brain ALDH2 activity than with liver ALDH2 activity. PMID: 14690875
    30. mtALDH overexpression attenuates hyperoxia-induced cell death in lung epithelial cells through reduction of reactive oxygen species, activation of ERK/MAPK, and PI3K-Akt cell survival signaling pathways PMID: 16782756
    31. lipoic acid has a role in the redox regulation of mitochondrial aldehyde dehydrogenase (ALDH-2) activity PMID: 17102135
    32. Increased superoxide production in nitrate tolerance is associated with Nox1 and Aldh2 down-regulation. PMID: 17493633
    33. mitochondrial aldehyde dehydrogenase 2 (ALDH2)was identified as an enzyme whose activation correlates with reduced ischemic heart damage in rodent models PMID: 18787169

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  • 亚细胞定位：
    Mitochondrion matrix.
  • 蛋白家族：
    Aldehyde dehydrogenase family
  • 数据库链接：