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  4. Rat alcohol dehydrogenase,ADH ELISA Kit

Rat alcohol dehydrogenase,ADH ELISA Kit

  • 中文名称：
    大鼠乙醇脱氢酶(ADH)酶联免疫试剂盒
  • 货号：
    CSB-E11180r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat ADH ELISA Kit was designed for the quantitative measurement of Rat ADH protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

  • 别名：
    Adh1 ELISA Kit; Adh-1 ELISA Kit; Alcohol dehydrogenase 1 ELISA Kit; EC 1.1.1.1 ELISA Kit; Alcohol dehydrogenase A subunit ELISA Kit
  • 缩写：
    ADH
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat ADH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %86-95
    1:2Average %101
    Range %96-105
    1:4Average %93
    Range %88-97
    1:8Average %97
    Range %91-103
  • 回收率：
    The recovery of rat ADH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9689-99
    EDTA plasma (n=4)8880-95
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.312 2.456 2.384 2.237
    101.586 1.712 1.649 1.502
    51.009 1.054 1.032 0.885
    2.50.641 0.632 0.637 0.490
    1.250.412 0.435 0.424 0.277
    0.6250.287 0.274 0.281 0.134
    0.3120.211 0.218 0.215 0.068
    00.146 0.147 0.147
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 基因功能参考文献：
    1. study provides evidence that the simultaneous increase of liver ADH and a reduction of ALDH activity by gene transfer could constitute a potential therapeutic strategy for the treatment of alcoholism. PMID: 22214999
    2. rat hepatic Class I ADH gene expression is regulated in vivo by ethanol in a mechanism involving the C/EBP site and the enhancer binding proteins beta and gamma PMID: 12213809
    3. Results describe the expression patterns of alcohol dehydrogenases I, III, and IV in adult rat, mouse and human tissues using radioactive oligonucleotide in situ hybridization. PMID: 12631290
    4. activity and expression in gastrointestinal tissue homogenates PMID: 12787032
    5. class I alcohol dehydrogenase is induced by sterol regulatory element-binding protein-1 in rat liver PMID: 15123720
    6. Alcohol dehydrogenase PMID: 16109828
    7. Cellular expression of Adh1 in the GI tract was studied. PMID: 17257171
    8. A high ADH activity leads to low ethanol intake in animals. PMID: 17488809
    9. ADH class I gene is progesterone-responsive in the uterus. PMID: 18219182
    10. Results revealed that kidney alcohol dehydrogenase activities increased significantly after ethanol administration, but the electrophoretic pattern of alcohol dehydrogenase isoforms was unmodified. PMID: 16421892

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Zinc-containing alcohol dehydrogenase family, Class-I subfamily
  • 数据库链接：