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  4. Rat Visceral adipose-specific serine protease inhibitor,vaspin ELISA Kit

Rat Visceral adipose-specific serine protease inhibitor,vaspin ELISA Kit

  • 中文名称：
    大鼠内脏脂肪特异性丝氨酸蛋白酶抑制剂(vaspin)酶联免疫试剂盒
  • 货号：
    CSB-E09813r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    Visceral Adipose-Specific Serine Protease Inhibitor, Vaspin is a member of the serine protease inhibitor family which has insulin-sensitizing effects.The Rat Visceral Adipose-Specific Serine Protease Inhibitor, Vaspin ELISA Kit is an essential tool for researchers in the metabolism and cardiovascular field. This quantitative assay is designed to detect and measure the level of Visceral Adipose Tissue-Derived Serine Protease Inhibitor (Vaspin) in serum, plasma, and tissue homogenates of Rattus norvegicus (Rat).

    The assay principle of this kit is based on the sandwich method, which ensures accurate and reliable results. The detection range of the Rat Vaspin ELISA Kit is 31.25 pg/mL-2000 pg/mL, and the sensitivity is 7.8 pg/mL. The assay time for this kit is 1-5 hours, and the sample volume required is only 50-100 ul.

    This kit's measurement is conducted at a detection wavelength of 450 nm, allowing for precise and efficient detection of Vaspin levels. Researchers can confidently use this kit to investigate the potential role of Vaspin in metabolism and cardiovascular diseases, given its specificity for visceral adipose tissue.

  • 别名：
    Serpina12 ELISA Kit; Serpin A12 ELISA Kit; Visceral adipose tissue-derived serine protease inhibitor ELISA Kit; Vaspin ELISA Kit; Visceral adipose-specific serpin ELISA Kit
  • 缩写：
    SERPINA12
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat vaspin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %85
    Range %80-90
    1:2Average %94
    Range %90-98
    1:4Average %91
    Range %85-97
    1:8Average %97
    Range %92-104
  • 回收率：
    The recovery of rat vaspin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 105100-110
    EDTA plasma (n=4)9590-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.679 2.576 2.628 2.479
    10002.071 2.114 2.093 1.944
    5001.582 1.472 1.527 1.378
    2500.958 0.913 0.936 0.787
    1250.580 0.589 0.585 0.436
    62.50.412 0.407 0.410 0.261
    31.250.302 0.279 0.291 0.142
    00.147 0.151 0.149
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Adipokine that modulates insulin action by specifically inhibiting its target protease KLK7 in white adipose tissues.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Serpin family
  • 组织特异性：
    Expressed in visceral adipose tissues.
  • 数据库链接：