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  4. Rat Tripartite motif-containing protein 72(TRIM72) ELISA kit

Rat Tripartite motif-containing protein 72(TRIM72) ELISA kit

  • 中文名称：
    大鼠三结构域包含蛋白72(TRIM72)酶联免疫试剂盒
  • 货号：
    CSB-EL024511RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat TRIM72 ELISA Kit was designed for the quantitative measurement of Rat TRIM72 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.7 pg/mL.

  • 别名：
    Trim72 ELISA Kit; Mg53 ELISA Kit; Tripartite motif-containing protein 72 ELISA Kit; Mitsugumin-53 ELISA Kit; Mg53 ELISA Kit
  • 缩写：
    TRIM72
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    18.75 pg/mL-1200 pg/mL
  • 灵敏度：
    4.7 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TRIM72 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %80-92
    1:2Average %97
    Range %91-105
    1:4Average %99
    Range %92-110
    1:8Average %93
    Range %86-98
  • 回收率：
    The recovery of rat TRIM72 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)9690-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    12002.814 2.989 2.902 2.817
    6002.366 2.441 2.404 2.319
    3001.559 1.457 1.508 1.423
    1500.753 0.697 0.725 0.640
    750.469 0.442 0.456 0.371
    37.50.243 0.235 0.239 0.154
    18.750.149 0.139 0.144 0.059
    00.088 0.082 0.085 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles.
  • 基因功能参考文献：
    1. These results suggest that an 8-week swimming exercise improves HFD-induced insulin resistance maybe through a reduction of TRIM72 in skeletal muscle and enhancement of AKT signal transduction. PMID: 27843952
    2. MG53 is an effective biomarker of myocardial injury and dysfunction in murine hearts. However, MG53 is not expressed in human heart and therefore does not hold utility as a clinical biomarker of myocardial injury PMID: 26790476
    3. MG53 is a facilitator of rapid injury repair, a mediator of cell migration, and a modulator of myofibroblast differentiation during wound healing PMID: 26306047
    4. MG53, a member of the muscle-specific transmembrane adaptor molecule (TRIM) protein family, is a crucial component of the cardiac ischemic preconditioning response. PMID: 20516375
  • 亚细胞定位：
    Cell membrane, sarcolemma. Cytoplasmic vesicle membrane.
  • 蛋白家族：
    TRIM/RBCC family
  • 数据库链接：