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  4. Rat Translationally-controlled tumor protein(TPT1) ELISA kit

Rat Translationally-controlled tumor protein(TPT1) ELISA kit

  • 中文名称：
    大鼠翻译调节肿瘤蛋白(TPT1)酶联免疫试剂盒
  • 货号：
    CSB-EL024134RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat TPT1 ELISA Kit was designed for the quantitative measurement of Rat TPT1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 39 pg/mL-2500 pg/mL and the sensitivity is 9.75 pg/mL .

  • 别名：
    Tpt1 ELISA Kit; Trt ELISA Kit; Translationally-controlled tumor protein ELISA Kit; TCTP ELISA Kit; Lens epithelial protein ELISA Kit
  • 缩写：
    TPT1
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    39 pg/mL-2500 pg/mL
  • 灵敏度：
    9.75 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TPT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %84
    Range %80-88
    1:2Average %94
    Range %90-98
    1:4Average %98
    Range %94-104
    1:8Average %95
    Range %88-99
  • 回收率：
    The recovery of rat TPT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9889-104
    EDTA plasma (n=4)9490-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    25002.047 2.134 2.091 1.959
    12501.384 1.483 1.434 1.302
    6250.854 0.836 0.845 0.713
    312.50.517 0.524 0.521 0.389
    1560.337 0.346 0.342 0.210
    780.257 0.248 0.253 0.121
    390.195 0.201 0.198 0.066
    00.134 0.129 0.132 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Involved in calcium binding and microtubule stabilization.
  • 基因功能参考文献：
    1. Tumor protein translationally-controlled -induced dopamine release from neuronal PC12 cells was modulated by a Ca(2+)-independent mechanism that involved PLA in the process, suggesting the regulatory role of TCTP in the neuronal functions. PMID: 27783042
    2. After acute spinal cord injury, TCTP in astrocytes gradually increased, peaking at day 3 and returning to basal levels by day 14. TCTP knockdown reduced astrocyte migration and proliferation. PMID: 26266488
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    TCTP family
  • 数据库链接：