Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Rat Tissue-type Plasiminogen Actilyse,t-PA ELISA Kit

Rat Tissue-type Plasiminogen Actilyse,t-PA ELISA Kit

  • 中文名称：
    大鼠组织型纤溶酶原激活剂(t-PA)酶联免疫试剂盒
  • 货号：
    CSB-E07917r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat PLAT ELISA Kit was designed for the quantitative measurement of Rat PLAT protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

  • 别名：
    Plat ELISA Kit; Tissue-type plasminogen activator ELISA Kit; t-PA ELISA Kit; t-plasminogen activator ELISA Kit; tPA ELISA Kit; EC 3.4.21.68) [Cleaved into: Tissue-type plasminogen activator chain A; Tissue-type plasminogen activator chain B] ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat t-PA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %85-97
    1:2Average %95
    Range %91-99
    1:4Average %96
    Range %89-103
    1:8Average %100
    Range %94-105
  • 回收率：
    The recovery of rat t-PA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10196-106
    EDTA plasma (n=4)9490-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    201.946 1.846 1.896 1.753
    101.542 1.440 1.491 1.348
    51.019 0.989 1.004 0.861
    2.50.536 0.529 0.533 0.390
    1.250.330 0.320 0.325 0.182
    0.6250.278 0.268 0.273 0.130
    0.3120.205 0.201 0.203 0.060
    00.144 0.142 0.143 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

平均分:
5.0分 - 1 个评价

样品类型：血浆（抗凝剂） edta

样品信息：大鼠

稀释比：没有稀释

产品评价： 我用RAT tPA elisa试剂盒检测大鼠血浆样本，复孔间数据稳定，但不稀释的情况下，整体OD偏低，灵敏度有待提高

By 杜老师

问答及客户评论

■ 常见问题解答
Q:

Is the kit of tPA(tissue plasminogen activator) cab be used for kidney rat lysat?
The research is about preeclampsia and damage to the kidney in pregnancy. We have a material that raises the level of tPA. tPA is anti coagulation protien that we think is involved in antiapoptosis.
We injected this material to pregnancy rats with preeclampsia, and we took out the kidneys. Now we want to test the level of tPa and its inhibitor PAI1, in this kidneys.
Please email me the product number?

A:
Thanks for your inquiry.
We have this kit for you: CSB-E07917r,Rat Tissue-type Plasiminogen Actilyse,t-PA ELISA Kit.
Additionally, if you want to test PAI1,we have this kit available for you: CSB-E07948r,Rat plasminogen activator inhibitor 1,PAI1 ELISA Kit.
I will send you the kit manuals by email.
Regarding to the 2 cases you mentioned, sorry we did not validate before.We suggest you do a pretest first.
If further questions,pls let us know.

靶点详情

  • 功能：
    Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. During oocyte activation, plays a role in cortical granule reaction in the zona reaction, which contributes to the block to polyspermy.
  • 基因功能参考文献：
    1. t-PA expressed in ECs may help to form IT of the DA via activation of MMP-2 and disruption of IEL PMID: 29304073
    2. LRP1 assembles unique co-receptor systems to initiate cell signaling in response to tissue-type plasminogen activator and myelin-associated glycoprotein. PMID: 24129569
    3. Regulation of PAI-1 activity in astrocytes by valproic acid may affect both physiological and pathological processes in the brain by upregulating tPA activity. PMID: 23378038
    4. Together, because CPAI blocks both proteolytic and nonproteolytic tPA neurotoxicity, it is a promising therapeutics of neonatal HI injury either with or without infection. PMID: 22556277
    5. Designed t-PA mutants with molecular properties that, in contrast to t-PA, do not induce neurotoxicity. PMID: 23301636
    6. Tissue plasminogen activator prevents mortality from sulfur mustard analog-induced airway obstruction. PMID: 23258228
    7. NR2B as a novel proteolytic substrate of tPA, where tPA may directly interact with NR2B subunits leading to a change in pharmacological properties of NR2B-containing NMDA receptors. PMID: 22610100
    8. mRNA, protein and activity of matrix metalloproteinase (MMP)-2 and MMP-9 and mRNA of tissue plasminogen activator (t-PA) and plasminogen activator inhibitor 1 (PAI-1) were quantified in abdominal aorta (AA) and femoral artery (FA). PMID: 22071631
    9. The induction of tPA by human marrow stromal cells after traumatic brain injury may be one of the mechanisms involved in promoting functional improvement after traumatic brain injury. PMID: 21355820
    10. Hydrocortisone up-regulates PAI-1 expression in a tissue-specific manner along with down-regulation of tPA activity in both normal and inflammatory conditions. PMID: 21412817
    11. PACAP stimulates the release of tPA which promotes cerebellar granule cell survival by a mechanism dependent upon its proteolytic activity. PMID: 21919910
    12. Massive up-regulation of plasminogen activator inhibitor-1 (PAI-1) in astrocytes during subchronic and chronic inflammatory conditions leads to decreased tPA activity in the later stages of reperfusion injury. PMID: 21193004
    13. Erigeron breviscapus injection can inhibit the expression of TNF-alpha and PAI-1, and increase the expression of tPA in rats with acute myocardial infarction. PMID: 21355198
    14. Investigated the brain-protective molecular mechanism of TPA in rats. TPA derivates were intracerebroventricularly administered at 15 min before, and 15, 90, 120 min after middle cerebral artery occlusion. PMID: 20596602
    15. Annexin A2 combined with low-dose tPA improves thrombolytic therapy in a rat model of focal embolic stroke. PMID: 20068577
    16. alpha-synuclein up-regulated MMP-9 and down-regulated tPA activity in rat primary astrocytes and microglia in ERK1/2 dependent fashion; suggests a role of alpha-synuclein via regulation of protease systems through modulation of ERK1/2 activity in brain PMID: 20026244
    17. Both annexin II and TPA were detected on the plasma membrane in a study of the role of proteases in fibronectin matrix remodeling in thyroid epithelial cell monolayer cultures as cells achieve confluence (annexin II)(tissue plasminogen activator) PMID: 11928811
    18. a chemical sympathectomy study of regulation of the vessel wall release of tissue plasminogen activator by sympathetic nervous system neurons PMID: 12192298
    19. Reciprocal actions of neural cell adhesion molecule and tissue plasminogen activator via a Ras-dependent mitogen-activated protein kinase signaling pathway in hippocampal neurons. PMID: 12387826
    20. Enhanced release of enzymatically active tissue plasminogen activator from sympathetic neurons into artery walls and the blood accompanies the changes associated with vascular aging. PMID: 12548713
    21. Low to moderate doses of rt-PA protect the microvascular basal lamina lamina during experimental focal cerebral ischemia, whereas high doses of rt-PA have the opposite effect, probably due to increased coactivation of MMP-2 and MMP-9. PMID: 12783121
    22. after transient focal cerebral ischemia, there are time dependent changes in tPA expression PMID: 14512838
    23. The 600 bp of the tPA promoter upstream of the transcription start site conveys cell specificity to tPA expression and an NF1 site within this region acts as a repressor. PMID: 15044208
    24. tPA activity is rapidly increased in hippocampal neurons after glutamate stimulation, corresponding to an increase in translational activation of mRNA present at the time of stimulation. PMID: 15496678
    25. These results suggest the importance of the t-PA/plasmin cascade during the early pathological stages of delayed neuronal death in the hippocampus following transient forebrain ischemia. PMID: 15882815
    26. Sympathetic nerves are the primary source of stress-induced release of t-PA into and from the densely innervated resistance arteries and arterioles. PMID: 16019605
    27. tPA upregulates brain MMP-9 levels in stroke in vivo PMID: 16051896
    28. tPA is localized in dendrites and terminals of AVP-expressing neurons; tPA is released in an activity-dependent manner, suggesting that matrix-degrading proteases are candidate molecules to be concerned with the structural plasticity in the HNS. PMID: 16150423
    29. tPA acts as a cytokine that binds to the cell membrane receptor LRP-1, induces its tyrosine phosphorylation, and triggers intracellular signal transduction to induce MMP9 gene expression PMID: 16303771
    30. TPA resides in dense-core granules that traffic to postsynaptic dendritic spines and that can remain there for extended periods. Depolarization by potassium elicits exocytotic release of tPA from DCGs in spines that is dependent on calcium signaling. PMID: 16555239
    31. Astrocyte-derived tPA and the proteolytic activity in the dorsal horn may be one of the essential factors involved in pain following root injury. PMID: 17299772
    32. Sertoli cells isolated from rat testes at various ages in vitro secreted tPA in an age-dependent manner. PMID: 17992606
    33. tPA induces LRP-1 tyrosine phosphorylation, which in turn facilitates the LRP-1-mediated recruitment of beta1 integrin and downstream ILK signaling, thereby leading to myofibroblast activation. PMID: 18037995
    34. The critical period of visual cortical plasticity, which occurs in early postnatal life, correlates well with tPA expression in the rat visual cortex. PMID: 18523654
    35. function of the tPA during diapedesis of monocytes through brain endothelial barrier; monocytes induce the release of tPA by brain endothelial cells; tPA and ERK1/2 control the breakdown of the tight junction protein occludin. PMID: 18714030
    36. The increase of tPA in the acute phase after hypoxic-ischemic brain damage may be helpful to clot dissolving, but it induces neuronal apoptosis and aggravates brain injury. PMID: 18947492
    37. FSH-induced ERK1/2 and p38 MAPK activation is capable of regulating tPA production in cultured primary granulosa cells. PMID: 18982462
    38. Our results demonstrated the involvement of tPA in the secondary pathogenesis after spinal cord injury as well as the therapeutic potential of hUCB. PMID: 19152029
    39. t-PA and PAI-1 mRNA were decreased after sympathectomy and remained unchanged after AT1 receptors blockade PMID: 19403340
    40. protein aggregates formed during neuronal injury provide a macromolecular, nonfibrin cofactor that promotes tPA-mediated plasmin formation and subsequent cell breakdown PMID: 19584397

    显示更多

    收起更多

  • 亚细胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    Peptidase S1 family
  • 数据库链接：