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  4. Rat Tissue factor pathway inhibitor,TFPI ELISA Kit

Rat Tissue factor pathway inhibitor,TFPI ELISA Kit

  • 中文名称：
    大鼠组织因子途径抑制物(TFPI)酶联免疫试剂盒
  • 货号：
    CSB-E08261r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat TFPI ELISA Kit was designed for the quantitative measurement of Rat TFPI protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 ng/mL-500 ng/mL and the sensitivity is 1.95 ng/mL.

  • 别名：
    Tfpi ELISA Kit; Tissue factor pathway inhibitor ELISA Kit; TFPI ELISA Kit; Extrinsic pathway inhibitor ELISA Kit; EPI ELISA Kit; Lipoprotein-associated coagulation inhibitor ELISA Kit; LACI ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    7.8 ng/mL-500 ng/mL
  • 灵敏度：
    1.95 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TFPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 85-96  
    1:2 Average % 89  
    Range % 84-95  
    1:4 Average % 93  
    Range % 89-97  
    1:8 Average % 90  
    Range % 85-94  
  • 回收率：
    The recovery of rat TFPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 100 95-107  
    EDTA plasma (n=4) 92 88-100  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    500 2.596 2.474 2.535 2.424  
    250 1.978 1.902 1.940 1.829  
    125 1.172 1.096 1.134 1.023  
    62.5 0.687 0.702 0.695 0.584  
    31.2 0.401 0.424 0.413 0.302  
    15.6 0.282 0.291 0.287 0.176  
    7.8 0.182 0.175 0.179 0.068  
    0 0.113 0.108 0.111    
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
  • 基因功能参考文献：
    1. TFPI expression is induced in cardiomyocytes, but not in endothelial cells, by IL-1beta. PMID: 11816723
    2. Rapid atrial pacing acutely downregulated the gene expression of thrombomodulin and TFPI in rat atrial endocardium, thereby inducing local coagulation imbalance on the internal surface of the atrial cavity PMID: 14610015
    3. TFPI levels decreased significantly at 24 hours in burn, septic injury, and burn+septic injury groups, followed by incomplete rebound recovery at 72 hours PMID: 15497025
    4. This is the first report showing that direct inhibition of TF-mediated coagulation activation abrogates bleomycin-induced pulmonary fibrosis. PMID: 16338226
    5. analysis of tissue factor changes and the effects of tissue factor pathway inhibitor on transient focal cerebral ischemia in rats PMID: 18067952
    6. The results demonstrated that overexpression of TFPI gene might induce VSMC apoptosis in vitro through the mitochondrial pathway; meanwhile, IAP-1 expression is decreased. PMID: 18368493

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  • 亚细胞定位：
    Secreted.
  • 组织特异性：
    Most abundant in heart, lung, kidney, and aortic endothelial cells.
  • 数据库链接：