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  4. Rat Tissue factor,TF ELISA Kit

Rat Tissue factor,TF ELISA Kit

  • 中文名称：
    大鼠组织因子(TF)酶联免疫试剂盒
  • 货号：
    CSB-E07914r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat tissue factor (TF) ELISA Kit was designed for the quantitative measurement of Rat tissue factor (TF) protein in serum, urine, cell culture supernates, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL .

  • 别名：
    F3 ELISA Kit; Cf3 ELISA Kit; Tissue factor ELISA Kit; TF ELISA Kit; Coagulation factor III ELISA Kit; CD antigen CD142 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, urine, cell culture supernates, tissue homogenates, cell lysates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Blood Coagulation
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %102
    Range %91-106
    1:2Average %90
    Range %85-96
    1:4Average %93
    Range %89-100
    1:8Average %99
    Range %93-108
  • 回收率：
    The recovery of rat TF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8884-94
    EDTA plasma (n=4)10395-107
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.297 2.231 2.264 2.081
    10001.573 1.471 1.522 1.339
    5000.999 0.924 0.962 0.779
    2500.618 0.593 0.606 0.423
    1250.405 0.425 0.415 0.232
    62.50.293 0.310 0.302 0.119
    31.250.249 0.264 0.257 0.074
    00.177 0.188 0.183 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Tissue factor family
  • 数据库链接：