Rat Tenascin-C(TN-C) ELISA Kit

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产品详情

产品描述：

This Rat TN-C ELISA Kit was designed for the quantitative measurement of Rat TN-C protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 2.5 ng/mL-160 ng/mL and the sensitivity is 0.625 ng/mL.
缩写：

TN-C
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

2.5 ng/mL-160 ng/mL
灵敏度：

0.625 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TN-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	84
	Range %	80-88
1:4	Average %	106
	Range %	101-113
1:8	Average %	93
	Range %	89-98

回收率：

The recovery of rat TN-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	88	84-92

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.