Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Rat Serine/threonine-protein kinase TNNI3K(TNNI3K) ELISA kit

Rat Serine/threonine-protein kinase TNNI3K(TNNI3K) ELISA kit

  • 中文名称：
    大鼠丝氨酸/苏氨酸蛋白激酶TNNI3K(TNNI3K)酶联免疫试剂盒
  • 货号：
    CSB-EL024014RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat TNNI3K ELISA Kit was designed for the quantitative measurement of Rat TNNI3K protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.69 pg/mL.

  • 别名：
    Tnni3k ELISA Kit; Cark ELISA Kit; Serine/threonine-protein kinase TNNI3K ELISA Kit; EC 2.7.11.1 ELISA Kit; Cardiac ankyrin repeat kinase ELISA Kit; TNNI3-interacting kinase ELISA Kit
  • 缩写：
    TNNI3K
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    18.75 pg/mL-1200 pg/mL
  • 灵敏度：
    4.69 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TNNI3K in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 91
    Range % 86-95
    1:2 Average % 102
    Range % 97-107
    1:4 Average % 91
    Range % 85-97
    1:8 Average % 97
    Range % 91-103
  • 回收率：
    The recovery of rat TNNI3K spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 89-98
    EDTA plasma (n=4) 97 90-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1200 2.465 2.561 2.513 2.401
    600 1.932 1.892 1.912 1.800
    300 1.277 1.298 1.288 1.176
    150 0.708 0.739 0.724 0.612
    75 0.412 0.432 0.422 0.310
    37.5 0.287 0.275 0.281 0.169
    18.75 0.183 0.185 0.184 0.072
    0 0.111 0.113 0.112 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    May play a role in cardiac physiology.
  • 基因功能参考文献：
    1. TNNI3K may be a novel mediator of cardiac troponin I phosphorylation and contribute to the regulation of cardiac myofilament contraction function. PMID: 23369981
    2. TNNI3K promotes a concentric hypertrophy with enhancement of cardiac function via regulating the phosphorylation of cTnI PMID: 23472207
  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    Protein kinase superfamily, TKL Ser/Thr protein kinase family, MAP kinase kinase kinase subfamily
  • 数据库链接：